Mutagenic activity of sweepings and pigments from a household-wax factory assayed with Salmonella typhimurium

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dc.contributor.authorVarella, S. D.
dc.contributor.authorPozetti, G. L.
dc.contributor.authorVilegas, Wagner [UNESP]
dc.contributor.authorVaranda, Eliana Aparecida [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T15:21:36Z
dc.date.available2014-05-20T15:21:36Z
dc.date.issued2004-12-01
dc.description.abstractThe mutagenic activity of garbage originating from a household wax industry was determined by the Salmonella/microsome assay, using the bacterial strains TA100, TA98 and YG1024. The garbage was obtained by sweeping the floor of the factory at the end of the work shift. Organic compounds were extracted by ultrasound for 30 min in dichloromethane or 70% ethanol. After evaporation of solvent, these extracts (HFS: household-wax factory sweepings) were dissolved in DMSO, and were tested for the mutagenic activity at varying concentrations (HFS-ET: 0.08-0.68 mg/plate, HFS-DCM: 0.60-7.31 mg/plate). The colouring agents (pigments) used in the production of the wax were also dissolved in DMSO and tested with the assay. The concentrations tested for each pigment were: Amaranth: 0.46-3.65 mg/plate, Auramine: 0.15-1.2 mg/plate, Tartrazine: 0.46-3.65 mg/plate and Rhodamine B: 0.22-1.82 mg/plate. Both ET and DCM organic extracts had mutagenic activity, especially in the YG1024 strain. The pigments behaved in a similar way, demonstrating that YG1024 was the most sensitive strain for the detection of mutagenicity, and that metabolization increased the activity. Human exposure (occupational and non-occupational) to industrial residues generated during the household-wax manufacturing and packaging process should be monitored, since this type of garbage is normally deposited in the environment without any control. (C) 2004 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Fac Pharmaceut Sci, Dept Biol Sci, BR-14801902 São Paulo, Brazil
dc.description.affiliationUniv Estadual Paulista, Inst Chem Araraquara, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Pharmaceut Sci, Dept Biol Sci, BR-14801902 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Chem Araraquara, BR-14801902 São Paulo, Brazil
dc.format.extent2029-2035
dc.identifierhttp://dx.doi.org/10.1016/j.fct.2004.07.019
dc.identifier.citationFood and Chemical Toxicology. Oxford: Pergamon-Elsevier B.V., v. 42, n. 12, p. 2029-2035, 2004.
dc.identifier.doi10.1016/j.fct.2004.07.019
dc.identifier.issn0278-6915
dc.identifier.lattes7927877224326837
dc.identifier.orcid0000-0003-3032-2556
dc.identifier.urihttp://hdl.handle.net/11449/32721
dc.identifier.wosWOS:000225252500013
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofFood and Chemical Toxicology
dc.relation.ispartofjcr3.977
dc.relation.ispartofsjr1,144
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectmutagenic activitypt
dc.subjectSalmonella typhimuriumpt
dc.subjectindustrial garbage mutagenicitypt
dc.subjectpigmentspt
dc.titleMutagenic activity of sweepings and pigments from a household-wax factory assayed with Salmonella typhimuriumen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.author.lattes7927877224326837
unesp.author.orcid0000-0003-3032-2556[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, São Vicentept

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Artigos - Ciências Biológicas - São Vicente