A survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormones

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dc.contributor.authorPalberg, Daniel
dc.contributor.authorKisiała, Anna
dc.contributor.authorJorge, Gabriel Lemes [UNESP]
dc.contributor.authorEmery, R. J. Neil
dc.contributor.institutionTrent University
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:50:42Z
dc.date.available2022-04-28T19:50:42Z
dc.date.issued2022-12-01
dc.description.abstractBackground: Symbiotic Methylobacterium strains comprise a significant part of plant microbiomes. Their presence enhances plant productivity and stress resistance, prompting classification of these strains as plant growth-promoting bacteria (PGPB). Methylobacteria can synthesize unusually high levels of plant hormones, called cytokinins (CKs), including the most active form, trans-Zeatin (tZ). Results: This study provides a comprehensive inventory of 46 representatives of Methylobacterium genus with respect to phytohormone production in vitro, including 16 CK forms, abscisic acid (ABA) and indole-3-acetic acid (IAA). High performance-liquid chromatography—tandem mass spectrometry (HPLC–MS/MS) analyses revealed varying abilities of Methylobacterium strains to secrete phytohormones that ranged from 5.09 to 191.47 pmol mL−1 for total CKs, and 0.46 to 82.16 pmol mL−1 for tZ. Results indicate that reduced methanol availability, the sole carbon source for bacteria in the medium, stimulates CK secretion by Methylobacterium. Additionally, select strains were able to transform L-tryptophan into IAA while no ABA production was detected. Conclusions: To better understand features of CKs in plants, this study uncovers CK profiles of Methylobacterium that are instrumental in microbe selection for effective biofertilizer formulations.en
dc.description.affiliationEnvironmental and Life Sciences Graduate Program Trent University, 1600 West Bank Drive
dc.description.affiliationDepartment of Biology Trent University, 1600 West Bank Drive
dc.description.affiliationDepartment of Technology Sao Paulo State University
dc.description.affiliationUnespDepartment of Technology Sao Paulo State University
dc.description.sponsorshipAgricultural Adaptation Council
dc.description.sponsorshipGenome Canada
dc.description.sponsorshipOntario Genomics Institute
dc.description.sponsorshipCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipNatural Sciences and Engineering Research Council of Canada
dc.description.sponsorshipGrain Farmers of Ontario
dc.description.sponsorshipIdAgricultural Adaptation Council: ON-RP3
dc.description.sponsorshipIdGenome Canada: ON-RP3
dc.description.sponsorshipIdOntario Genomics Institute: ON-RP3
dc.description.sponsorshipIdCanadian Network for Research and Innovation in Machining Technology, Natural Sciences and Engineering Research Council of Canada: RGPIN-2018-05436
dc.description.sponsorshipIdNatural Sciences and Engineering Research Council of Canada: RGPIN-2018-05436
dc.description.sponsorshipIdGrain Farmers of Ontario: S2013AG04
dc.identifierhttp://dx.doi.org/10.1186/s12866-022-02454-9
dc.identifier.citationBMC Microbiology, v. 22, n. 1, 2022.
dc.identifier.doi10.1186/s12866-022-02454-9
dc.identifier.issn1471-2180
dc.identifier.scopus2-s2.0-85124323314
dc.identifier.urihttp://hdl.handle.net/11449/223437
dc.language.isoeng
dc.relation.ispartofBMC Microbiology
dc.sourceScopus
dc.subject2-methylthio-Zeatin
dc.subjectHPLC–MS/MS
dc.subjectMethanol
dc.subjectPlant growth promoting bacteria
dc.subjecttrans-Zeatin
dc.titleA survey of Methylobacterium species and strains reveals widespread production and varying profiles of cytokinin phytohormonesen
dc.typeArtigo
unesp.author.orcid0000-0001-5966-546X[1]
unesp.author.orcid0000-0002-4918-7836[2]
unesp.author.orcid0000-0003-3541-5129[3]
unesp.author.orcid0000-0003-1304-6430[4]

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