Antimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro study

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dc.contributor.authorSangalli, Jorgiana [UNESP]
dc.contributor.authorJardim Júnior, Elerson Gaetti [UNESP]
dc.contributor.authorBueno, Carlos Roberto Emerenciano [UNESP]
dc.contributor.authorJacinto, Rogério Castilho [UNESP]
dc.contributor.authorSivieri-Araújo, Gustavo [UNESP]
dc.contributor.authorFilho, João Eduardo Gomes [UNESP]
dc.contributor.authorCintra, Luciano Tavares Ângelo [UNESP]
dc.contributor.authorDezan Junior, Eloi [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:17:16Z
dc.date.available2018-12-11T17:17:16Z
dc.date.issued2018-07-01
dc.description.abstractObjective Evaluate, in vitro, the antimicrobial activity of Psidium cattleianum leaf extracts combined with calcium hydroxide against Enterococcus faecalis and Candida albicans biofilm. Materials and methods Dentin specimens obtained from extracted bovine incisors were infected during 14 days with E. faecalis ATCC 29212 and C. albicans ATCC 10231. The specimens were filled with calcium hydroxide pastes prepared with the following vehicles: Psidium cattleianum ethanolic, Psidium cattleianum propylene glycolic, distilled water, and saline as control. After 24 h, 3, 7, and 14 days, the canals were irrigated with sterile saline and dried. Dentin samples were collected from the canals with burs of increasing diameters. To determine the number of colony-forming units (CFU), samples were inoculated onto BHI agar supplemented with yeast extract (0.5%), at 37 °C, for 48 h, in CO2 enriched atmosphere. Comparisons among the groups for the variation factors were performed by ANOVA and Tukey’s test. Results Ethanolic and propylene glycolic extracts showed significantly higher antimicrobial activity against E. faecalis (p <0.01) when compared with distilled water. The ethanolic extract exhibited in 24 h the same antibacterial activity that propylene glycolic extract and distilled water after 7 and 14 days. For C. albicans, all were effective in reducing the number of CFU at all periods. Conclusion The P. cattleianum ethanolic extract presented the fastest and highest antimicrobial activity against E. faecalis, significantly reducing the microbial load in 24 h. All medications were effective against C. albicans. Clinical relevance The antibacterial potential of P. cattleianum and its biological compatibility associated with calcium hydroxide indicate promising applications in the field of dentistry.en
dc.description.affiliationDepartment of Restorative Dentistry Endodontics Araçatuba School of Dentistry UNESP Univ Estadual Paulista, Rua José Bonifácio, 1193
dc.description.affiliationDepartment of Pathology Microbiology and Imunology Araçatuba School of Dentistry UNESP Univ Estadual Paulista
dc.description.affiliationUnespDepartment of Restorative Dentistry Endodontics Araçatuba School of Dentistry UNESP Univ Estadual Paulista, Rua José Bonifácio, 1193
dc.description.affiliationUnespDepartment of Pathology Microbiology and Imunology Araçatuba School of Dentistry UNESP Univ Estadual Paulista
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent2273-2279
dc.identifierhttp://dx.doi.org/10.1007/s00784-017-2326-5
dc.identifier.citationClinical Oral Investigations, v. 22, n. 6, p. 2273-2279, 2018.
dc.identifier.doi10.1007/s00784-017-2326-5
dc.identifier.file2-s2.0-85040240667.pdf
dc.identifier.issn1436-3771
dc.identifier.issn1432-6981
dc.identifier.lattes8813777272581972
dc.identifier.lattes3953630077047017
dc.identifier.orcid0000-0002-5699-6578
dc.identifier.orcid0000-0002-2362-8920
dc.identifier.scopus2-s2.0-85040240667
dc.identifier.urihttp://hdl.handle.net/11449/175731
dc.language.isoeng
dc.relation.ispartofClinical Oral Investigations
dc.relation.ispartofsjr0,986
dc.relation.ispartofsjr0,986
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectCalcium hydroxide
dc.subjectCandida albicans
dc.subjectEnterococcus faecalis
dc.subjectPlant extracts
dc.titleAntimicrobial activity of psidium cattleianum associated with calcium hydroxide against enterococcus faecalis and candida albicans: An in vitro studyen
dc.typeArtigo
unesp.author.lattes8813777272581972[8]
unesp.author.lattes3953630077047017[4]
unesp.author.orcid0000-0002-5699-6578[8]
unesp.author.orcid0000-0002-2362-8920[4]

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Artigos - Odontologia Restauradora - FOA
Artigos - Patologia e Propedêutica Clínica - FOA