Plasma Steroid Dynamics in Late- and Near-term Naturally and Artificially Conceived Bovine Pregnancies as Elucidated by Multihormone High-resolution LC-MS/MS

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dc.contributor.authorMartins-Junior, Helio A.
dc.contributor.authorPinaffi, Fabio L. V.
dc.contributor.authorSimas, Rosineide C. [UNESP]
dc.contributor.authorTarouco, Adriana K.
dc.contributor.authorFerreira, Christina R.
dc.contributor.authorSilva, Luciano A.
dc.contributor.authorNogueira, Guilherme P. [UNESP]
dc.contributor.authorMeirelles, Flavio V.
dc.contributor.authorEberlin, Marcos N.
dc.contributor.authorPerecin, Felipe
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionAB SCIEX Brazil
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionState Fdn Agr Res
dc.date.accessioned2015-11-03T15:27:56Z
dc.date.available2015-11-03T15:27:56Z
dc.date.issued2014-12-01
dc.description.abstractThe plasma levels of corticosteroids and sex steroids during pregnancy are key indicators of mammalian placental function and the onset of parturition. Steroid hormones are believed to be disturbed in pregnancies produced using assisted reproductive technologies (ARTs) due to placental dysfunction and the frequently observed lack of parturition signals. To elucidate the plasma steroid dynamics, a liquid chromatography-tandem mass spectrometry method was developed and used to determine the levels of corticosteroids (corticosterone, 11-deoxycortisol, and cortisol) and their direct precursors (progesterone and 17 alpha-OH-progesterone) as well as sex steroids (androstenedione, estrone, estrone sulfate, testosterone, and 17 beta-estradiol) in bovine plasma. The levels of these 10 steroids in recipient cows carrying naturally conceived (control), in vitro fertilized (IVF), or cloned (somatic cell nuclear transfer) conceptuses were compared during late-term pregnancy (30 days before parturition), during near-term pregnancy (1 day before parturition), and on the day of parturition (day 0). Significant differences were observed among the corticosteroid levels: higher levels of corticosterone, 11-deoxycortisol, and cortisol were detected in cloned pregnancies at day 30; lower levels of corticosterone were observed in ART-derived pregnancies at days 1 and 0; and estrone and estradiol levels were higher in IVF pregnancies through out the final development. These results suggested an upregulation of the P450(C11) and P450(C21) enzymes 30 days before parturition in somatic cell nuclear transfer pregnancies and an over-activation of the aromatase enzyme in IVF pregnancies. Taken together, the monitoring of multiple steroid hormones revealed that the pregnancies obtained using ART exhibited plasma steroid concentration dynamics compatible with the dysregulation of steroidogenic tissues.en
dc.description.affiliationUniv Estadual Campinas, Inst Chem, Thomson Mass Spectrometry Lab, Campinas, SP, Brazil
dc.description.affiliationAB SCIEX Brazil, BR-04719002 Sao Paulo, Brazil
dc.description.affiliationUniv Sao Paulo, Lab Theriogenol Dr OJ Ginther, Dept Vet Med, Sch Anim Sci &Food Engn FZEA, BR-13635900 Pirassununga, SP, Brazil
dc.description.affiliationUniv Sao Paulo, Lab Mol Morphophysiol &Dev, Dept Vet Med, Sch Anim Sci &Food Engn, BR-13635900 Pirassununga, SP, Brazil
dc.description.affiliationSao Paulo State Univ, Dept Prod &Anim Hlth, Sch Vet Med, BR-16050680 Aracatuba, SP, Brazil
dc.description.affiliationState Fdn Agr Res, Res Ctr Iwar Beckman, BR-96400970 Hulha Negra, RS, Brazil
dc.description.affiliationUnespSao Paulo State Univ, Dept Prod &Anim Hlth, Sch Vet Med, BR-16050680 Aracatuba, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipBrazilian research foundation National Council for Scientific and Technological Development
dc.description.sponsorshipIdFAPESP: 2007/52003-2
dc.description.sponsorshipIdFAPESP: 2007/58786-9
dc.description.sponsorshipIdFAPESP: 2010/05806-5
dc.description.sponsorshipIdFAPESP: 2010/51677-2
dc.description.sponsorshipIdFAPESP: 2010/15167-0
dc.format.extent5011-5023
dc.identifierhttp://press.endocrine.org/doi/abs/10.1210/en.2013-2166
dc.identifier.citationEndocrinology. Washington: Endocrine Soc, v. 155, n. 12, p. 5011-5023, 2014.
dc.identifier.doi10.1210/en.2013-2166
dc.identifier.issn0013-7227
dc.identifier.urihttp://hdl.handle.net/11449/129934
dc.identifier.wosWOS:000346668000038
dc.language.isoeng
dc.publisherEndocrine Soc
dc.relation.ispartofEndocrinology
dc.relation.ispartofjcr3.961
dc.relation.ispartofsjr1,878
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titlePlasma Steroid Dynamics in Late- and Near-term Naturally and Artificially Conceived Bovine Pregnancies as Elucidated by Multihormone High-resolution LC-MS/MSen
dc.typeArtigo
dcterms.rightsHolderEndocrine Soc
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária, Araçatubapt

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