Diversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazil

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dc.contributor.authorOliveira-Arbex, Ana Paula [UNESP]
dc.contributor.authorDavid, Érica Boarato [UNESP]
dc.contributor.authorTenório, Michely da Silva [UNESP]
dc.contributor.authorCicchi, Paulo José Pyles [UNESP]
dc.contributor.authorPatti, Mariella
dc.contributor.authorCoradi, Silvana Torossian
dc.contributor.authorLucheis, Simone Baldini
dc.contributor.authorJim, Jorge [UNESP]
dc.contributor.authorGuimarães, Semíramis [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionIntegrated Faculties of Bauru (FIB)
dc.contributor.institutionSagrado Coração University (USC)
dc.contributor.institutionPaulista Agency of Agribusiness Technology (APTA/SAA)
dc.date.accessioned2020-12-12T01:44:51Z
dc.date.available2020-12-12T01:44:51Z
dc.date.issued2020-03-01
dc.description.abstractThe enteric protist Blastocystis is one of the most commonly parasite reported in humans and a variety of animal hosts worldwide. Regarding genetic diversity, at least 17 subtypes (STs) have been identified in mammals and birds, with eight of them (ST1-8) infecting both humans and animals. Recently, isolates from wild mammalian species have been genetically characterized, however data is still scarce, mainly in Latin America. Here, we aimed to verify the occurrence and genetic diversity of Blastocystis in captive wild mammals kept in one zoo and in two units of protection and conservation in southeastern Brazil. A total of 78 fecal samples (14 pooled and 64 individual samples) were recovered from 102 wild mammals of 35 species included in the following orders: Primates, Carnivora, Artiodactyla, Pilosa, Rodentia and Marsupialia. Zoo and units staff were invited to participated but only 16 fecal samples could be screened. Based on the sequence analyses of SSUrDNA gene, out of 29 PCR products from animal samples, 51.7% (15/29) were successfully sequenced and five Blastocystis subtypes were identified as follows: ST1 (2/15; 13.3%), ST2 (2/15; 13.3%), ST3 (4/15; 26.6%), ST5 (2/15; 13.3%) and ST8 (5/14; 33.3%). Only four isolates from humans were sequenced and identified as ST1 (2 isolates), ST2 and ST3. It was observed that Blastocystis infecting non-human primates belong to ST1 and ST2 and mainly to ST3 and ST8, artiodactyls ST5, carnivores ST1 and ST5 and rodents ST1. In addition, this present study reports some interesting findings: (1) 63% (12/19) of Blastocystis isolates from animals and employees belonged to the potentially zoonotic subtypes ST1-ST3; (2) most of these isolates displayed high identity with publicly available DNA sequences from non-human primates and humans, including primate handlers; (3) Blastocystis ST5 was found infecting the northern tiger cat, a native South American felid and one of the species facing a high risk of extinction in Brazil.en
dc.description.affiliationDepartment of Parasitology Institute of Bioscience São Paulo State University (UNESP)
dc.description.affiliationIntegrated Faculties of Bauru (FIB)
dc.description.affiliationSagrado Coração University (USC) Department of Health Sciences
dc.description.affiliationTropical Diseases Postgraduate Program Medical School São Paulo State University (UNESP)
dc.description.affiliationDepartment of Zoology Institute of Bioscience São Paulo State University (UNESP)
dc.description.affiliationPaulista Agency of Agribusiness Technology (APTA/SAA)
dc.description.affiliationUnespDepartment of Parasitology Institute of Bioscience São Paulo State University (UNESP)
dc.description.affiliationUnespTropical Diseases Postgraduate Program Medical School São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Zoology Institute of Bioscience São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1016/j.meegid.2019.104053
dc.identifier.citationInfection, Genetics and Evolution, v. 78.
dc.identifier.doi10.1016/j.meegid.2019.104053
dc.identifier.issn1567-7257
dc.identifier.issn1567-1348
dc.identifier.scopus2-s2.0-85074626837
dc.identifier.urihttp://hdl.handle.net/11449/199620
dc.language.isoeng
dc.relation.ispartofInfection, Genetics and Evolution
dc.sourceScopus
dc.titleDiversity of Blastocystis subtypes in wild mammals from a zoo and two conservation units in southeastern Brazilen
dc.typeArtigo

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