Differential Expression of IGF Family Members in Heat-Stressed Embryos Produced In Vitro from OPU-Derived Oocytes of Nelore (Bos indicus) and Holstein (Bos taurus) Cows

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dc.contributor.authorSatrapa, R. A. [UNESP]
dc.contributor.authorRazza, E. M. [UNESP]
dc.contributor.authorCastilho, A. C. S. [UNESP]
dc.contributor.authorSimoes, R. A. L. [UNESP]
dc.contributor.authorSilva, C. F. [UNESP]
dc.contributor.authorNabhan, T. [UNESP]
dc.contributor.authorPegorer, M. F. [UNESP]
dc.contributor.authorBarros, C. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:52Z
dc.date.available2014-12-03T13:10:52Z
dc.date.issued2013-12-01
dc.description.abstractContents The IGF system is related to embryo quality. We aim to determine the effect of the heat stress on the mRNA expression of IGF1 and IGF2, IGFR1 and IGFR2, IGFBP2 and IGFBP4, and PAPPA in in vitro production (IVP) blastocysts from Nelore and Holstein after ovum pick up (OPU) to better understand the differences between these breeds.Oocytes from four Nelore and seven Holstein were collected in six OPU sessions. Following in vitro maturation and fertilization using six Nelore or Holstein sires, embryos were divided into control (cultured at 39 degrees C) and heat stress (HS; exposed to 41 degrees C for 9h). Blastocysts were submitted to RNA extraction. The IGF1 expression was higher in blastocysts under HS in both breeds, and the expression of IGFBP2 and IGFBP4 was higher in Holstein blastocysts under HS. The high PAPPA expression and the low expression of IGFBP2 and IGFBP4 are associated with a more efficient degradation of IGFBPs, which results in greater IGF bioavailability in Nelore blastocysts and may contribute to the superior HS tolerance in Nelore, when compared to Holstein.en
dc.description.affiliationDepartment of Pharmacology, UNESP, Botucatu, Brazil
dc.description.affiliationDepartment of Animal Reproduction, UNESP, Botucatu, Brazil
dc.description.affiliationUnespDepartment of Pharmacology, UNESP, Botucatu, Brazil
dc.description.affiliationUnespDepartment of Animal Reproduction, UNESP, Botucatu, Brazil
dc.format.extent1043-1048
dc.identifierhttp://dx.doi.org/10.1111/rda.12211
dc.identifier.citationReproduction In Domestic Animals. Hoboken: Wiley-blackwell, v. 48, n. 6, p. 1043-1048, 2013.
dc.identifier.doi10.1111/rda.12211
dc.identifier.issn0936-6768
dc.identifier.lattes7795883009987806
dc.identifier.urihttp://hdl.handle.net/11449/112604
dc.identifier.wosWOS:000330107800028
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofReproduction in Domestic Animals
dc.relation.ispartofjcr1.422
dc.relation.ispartofsjr0,594
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleDifferential Expression of IGF Family Members in Heat-Stressed Embryos Produced In Vitro from OPU-Derived Oocytes of Nelore (Bos indicus) and Holstein (Bos taurus) Cowsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
unesp.author.lattes7795883009987806
unesp.author.orcid0000-0002-5193-0596[2]
unesp.author.orcid0000-0003-1666-7021[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina Veterinária e Zootecnia, Botucatupt

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Artigos - Farmacologia - IBB
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