Crystallization and preliminary X-ray diffraction analysis of three myotoxic phospholipases A2 from Bothrops brazili venom

Página do item simplificado
dc.contributor.authorFernandes, Carlos A. H. [UNESP]
dc.contributor.authorGartuzo, Elaine C. G. [UNESP]
dc.contributor.authorPagotto, Ivan [UNESP]
dc.contributor.authorComparetti, Edson J. [UNESP]
dc.contributor.authorHuancahuire-Vega, Salomon
dc.contributor.authorPonce-Soto, Luis Alberto
dc.contributor.authorCosta, Tassia R.
dc.contributor.authorMarangoni, Sergio
dc.contributor.authorSoares, Andreimar M.
dc.contributor.authorFontes, Marcos R. M. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Federal de Rondônia (UNIR)
dc.date.accessioned2014-05-20T13:49:41Z
dc.date.available2014-05-20T13:49:41Z
dc.date.issued2012-08-01
dc.description.abstractTwo myotoxic and noncatalytic Lys49-phospholipases A2 (braziliantoxin-II and MT-II) and a myotoxic and catalytic phospholipase A2 (braziliantoxin-III) from the venom of the Amazonian snake Bothrops brazili were crystallized. The crystals diffracted to resolutions in the range 2.562.05 angstrom and belonged to space groups P3121 (braziliantoxin-II), P6522 (braziliantoxin-III) and P21 (MT-II). The structures were solved by molecular-replacement techniques. Both of the Lys49-phospholipases A2 (braziliantoxin-II and MT-II) contained a dimer in the asymmetric unit, while the Asp49-phospholipase A2 braziliantoxin-III contained a monomer in its asymmetric unit. Analysis of the quaternary assemblies of the braziliantoxin-II and MT-II structures using the PISA program indicated that both models have a dimeric conformation in solution. The same analysis of the braziliantoxin-III structure indicated that this protein does not dimerize in solution and probably acts as a monomer in vivo, similar to other snake-venom Asp49-phospholipases A2.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP, Brazil
dc.description.affiliationCNPq, Inst Nacl Ciência & Tecnol Toxinas, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Inst Biol, Dept Bioquim, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Farmacol, Fac Ciencias Med, Campinas, SP, Brazil
dc.description.affiliationUniv São Paulo, Dept Anal Clin Toxicol & Bromatol, FCFRP, Ribeirao Preto, SP, Brazil
dc.description.affiliationUniv Fed Rondonia, Fundação Oswaldo Cruz, FIOCRUZ Rondonia, Porto Velho, RO, Brazil
dc.description.affiliationUniv Fed Rondonia, Ctr Estudos Biomol Aplicadas, Porto Velho, RO, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Inst Biociencias, Dept Fis & Biofis, Botucatu, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent935-938
dc.identifierhttp://dx.doi.org/10.1107/S1744309112026073
dc.identifier.citationActa Crystallographica Section F-structural Biology and Crystallization Communications. Hoboken: Wiley-blackwell, v. 68, p. 935-938, 2012.
dc.identifier.doi10.1107/S1744309112026073
dc.identifier.fileWOS000307217700019.pdf
dc.identifier.issn1744-3091
dc.identifier.urihttp://hdl.handle.net/11449/17711
dc.identifier.wosWOS:000307217700019
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Crystallographica Section F: Structural Biology and Crystallization Communications
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectphospholipases A2en
dc.subjectBothrops brazilien
dc.subjectbraziliantoxin-IIen
dc.subjectbraziliantoxin-IIIen
dc.subjectMT-IIen
dc.titleCrystallization and preliminary X-ray diffraction analysis of three myotoxic phospholipases A2 from Bothrops brazili venomen
dc.typeArtigo
dcterms.licensehttp://journals.iucr.org/services/copyrightpolicy.html
dcterms.rightsHolderWiley-blackwell
unesp.author.orcid0000-0001-5976-2913[6]
unesp.author.orcid0000-0002-4634-6221[10]
unesp.author.orcid0000-0001-6515-6872[1]
unesp.author.orcid0000-0002-4848-4767[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
WOS000307217700019.pdf
Tamanho:
282.07 KB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 2 de 2
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Física e Biofísica - IBB