Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus
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Data
2023-03-01
Autores
Bertolini, Amanda Bezerra [UNESP]
Thyssen, Patrícia Jacqueline
Brandão, Paulo Eduardo
Prado, Aline Marrara
Silva, Sheila Oliveira de Souza
Mioni, Mateus de Souza Ribeiro [UNESP]
de Gouvea, Fábio Lucas Rezende [UNESP]
Pantoja, José Carlos de Figueiredo [UNESP]
Langoni, Helio [UNESP]
Guimarães, Felipe Freitas [UNESP]
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Resumo
AIMS: We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms. METHODS AND RESULTS: We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified. Rotavirus A (RVA) and betacoronavirus (BCoV) were detected in 14.7% (32/217) and 4.6% (10/217) of the dipterans, respectively. Sequencing of the amplicons was possible for 11.5% (25/217) of RVA and 0.5% (1/217) of BCoV, confirming the presence of these pathogens. CONCLUSIONS: Our findings highlight the role of dipterans as carriers of RVA and BCoV of great relevance for public and animal health.
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BCoV, dairy cows, flying insects, RVA, Vector-borne diseases
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Journal of applied microbiology, v. 134, n. 3, 2023.