Detection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assay

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dc.contributor.authorKim, Yeon Jung [UNESP]
dc.contributor.authorViana, Aline Cavalcanti
dc.contributor.authorTfaile Corbi, Samia Cruz
dc.contributor.authorde Carvalho Curtis, Karen Maria [UNESP]
dc.contributor.authorRenzi, Rivelto
dc.contributor.authorOrrico, Silvana Regina Perez [UNESP]
dc.contributor.authorCirelli, Joni Augusto [UNESP]
dc.contributor.authorScarel-Caminaga, Raquel Mantuaneli [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2013-09-30T18:32:04Z
dc.date.accessioned2014-05-20T13:45:32Z
dc.date.available2013-09-30T18:32:04Z
dc.date.available2014-05-20T13:45:32Z
dc.date.issued2009-11-01
dc.description.abstractInterleukin 8 (IL-8) is a chemokine that acts as a potent chemoattractant for neutrophils. Single nucleotide polymorphisms ( SNPs) in the human IL8 gene have been investigated in many disease association studies. We have developed a different PCR-RFLP (Polymerase Chain Reaction - Restriction Fragment of Length Polymorphism) assay for genotyping the SNP (rs2227307) in the IL8 gene. This method was used for typing 147 white healthy Brazilian individuals, whose DNA was obtained from buccal epithelial cells and extracted with phenol: chloroform: isoamyl alcohol. Genomic DNA was amplified by PCR using a conventional thermal cycler. The PCR products ( 573 bp) were submitted to RFLP reactions. The RFLP fragments were analyzed in a 4% agarose gel stained with ethidium bromide. The genotype distribution observed in this study was consistent with the assumption of Hardy-Weinberg equilibrium and was similar (p=0.30) to those reported for other white populations in the SNP Database of the National Center for Biotechnology Information (NCBI). Because the PCR-RFLP method presented here was efficient, low cost, reproducible and convenient for laboratories with a limited level of technology worldwide, it should be useful for genotyping in case-control association or population genetic studies.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Fac Odontol Araraquara FOAr, Araraquara, SP, Brazil
dc.description.affiliationUNESP, Inst Quim Araraquara, Araraquara, SP, Brazil
dc.description.affiliationUNESP, FOAr, Disciplina Periodontia, Araraquara, SP, Brazil
dc.description.affiliationUNESP, FOAr, Disciplina Genet Humana, Araraquara, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Fac Odontol Araraquara FOAr, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Inst Quim Araraquara, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, FOAr, Disciplina Periodontia, Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, FOAr, Disciplina Genet Humana, Araraquara, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipIdFAPESP: 03/10424-0
dc.description.sponsorshipIdFAPESP: 05/03231-7
dc.description.sponsorshipIdFAPESP: 05/04553-8
dc.description.sponsorshipIdFAPESP: 06/04492-1
dc.format.extent136-142
dc.identifierhttp://www.seer.ufu.br/index.php/biosciencejournal/article/view/7041
dc.identifier.citationBioscience Journal. Uberlandia: Universidade Federal de Uberlândia (UFU), v. 25, n. 6, p. 136-142, 2009.
dc.identifier.fileWOS000272788800015.pdf
dc.identifier.issn1516-3725
dc.identifier.lattes7383391319292040
dc.identifier.lattes2628593693450121
dc.identifier.urihttp://hdl.handle.net/11449/16026
dc.identifier.wosWOS:000272788800015
dc.language.isoeng
dc.publisherUniversidade Federal de Uberlândia (UFU)
dc.relation.ispartofBioscience Journal
dc.relation.ispartofsjr0,303
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectSingle Nucleotide Polymorphismen
dc.subjectInterleukin 8en
dc.subjectPCR-RFLP assayen
dc.titleDetection of the single nucleotide polymorphism (rs2227307) in the human interleukin 8 gene using a pcr-rflp assayen
dc.typeArtigo
dcterms.licensehttp://www.seer.ufu.br/index.php/biosciencejournal/about/editorialPolicies#openAccessPolicy
dcterms.rightsHolderUniversidade Federal de Uberlândia (UFU)
unesp.author.lattes7383391319292040
unesp.author.lattes2628593693450121
unesp.author.orcid0000-0002-8235-6072[3]
unesp.author.orcid0000-0001-5068-0268[8]
unesp.author.orcid0000-0002-7082-9290[7]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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Artigos - Diagnóstico e Cirurgia - FOAR