Quantitative Proteomic Profiling Reveals That Diverse Metabolic Pathways Are Influenced by Melatonin in an in Vivo Model of Ovarian Carcinoma

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dc.contributor.authorChuffa, Luiz Gustavo A. [UNESP]
dc.contributor.authorLupi Júnior, Luiz Antonio [UNESP]
dc.contributor.authorSeiva, Fábio R. F.
dc.contributor.authorMartinez, Marcelo
dc.contributor.authorDomeniconi, Raquel F. [UNESP]
dc.contributor.authorPinheiro, Patricia Fernanda F. [UNESP]
dc.contributor.authorDos Santos, Lucilene D. [UNESP]
dc.contributor.authorMartinez, Francisco Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal de São Carlos (UFSCar)
dc.contributor.institutionCLM
dc.date.accessioned2018-12-11T17:06:35Z
dc.date.available2018-12-11T17:06:35Z
dc.date.issued2016-10-07
dc.description.abstractTo obtain more information into the molecular mechanisms underlying ovarian cancer (OC), we proposed a comparative proteomic analysis in animals receiving long-term melatonin as therapy or only vehicle using multidimensional protein identification combined with mass spectrometry. To induce tumor, a single dose of 100 μg 7,12-dimethylbenz(a)anthracene (DMBA) dissolved in 10 μL of sesame oil was injected under the left ovarian bursa of 20 Fischer 344 rats. The right ovaries were injected with sesame oil only. After tumors were developed, half of the animals received intraperitoneal administration of melatonin (200 μg/100g body weight/day) for 60 days. Melatonin therapy promoted down-regulation in numerous proteins involved in OC signaling pathways. The most significant portion of these proteins are involved in several metabolic processes, mainly those associated with mitochondrial systems, generation of metabolites and energy, hypoxia-inducible factor-1 signaling, antigen processing and presentation, endoplasmic reticulum stress-associated pathways, and cancer-related proteoglycans. A small number of proteins that were overexpressed by melatonin therapy included ATP synthase subunit β, fatty acid-binding protein, and 10-kDa heat shock protein. Taken together, our findings suggest that melatonin therapy efficiently modulated important signaling pathways involved in OC, and these proteins might be further targets that should be explored in new therapeutic opportunities for OC.en
dc.description.affiliationDepartment of Anatomy Institute of Biosciences UNESP Universidade Estadual Paulista
dc.description.affiliationDepartment of Morphology and Pathology UFSCar Universidade Federal de São Carlos
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) UNESP Univ Estadual Paulista
dc.description.affiliationInstitute of Biology North of Parana State University UENP CLM
dc.description.affiliationUnespDepartment of Anatomy Institute of Biosciences UNESP Universidade Estadual Paulista
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) UNESP Univ Estadual Paulista
dc.format.extent3872-3882
dc.identifierhttp://dx.doi.org/10.1021/acs.jproteome.6b00713
dc.identifier.citationJournal of Proteome Research, v. 15, n. 10, p. 3872-3882, 2016.
dc.identifier.doi10.1021/acs.jproteome.6b00713
dc.identifier.issn1535-3907
dc.identifier.issn1535-3893
dc.identifier.lattes5760560970751598
dc.identifier.lattes5481756528299469
dc.identifier.orcid0000-0003-1452-5708
dc.identifier.orcid0000-0003-2938-010X
dc.identifier.scopus2-s2.0-84990853375
dc.identifier.urihttp://hdl.handle.net/11449/173579
dc.language.isoeng
dc.relation.ispartofJournal of Proteome Research
dc.relation.ispartofsjr1,818
dc.relation.ispartofsjr1,818
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectenergy
dc.subjectER stress
dc.subjectmass spectrometry
dc.subjectmelatonin
dc.subjectmitochondrial metabolism
dc.subjectovarian cancer
dc.titleQuantitative Proteomic Profiling Reveals That Diverse Metabolic Pathways Are Influenced by Melatonin in an in Vivo Model of Ovarian Carcinomaen
dc.typeArtigo
unesp.author.lattes5760560970751598[6]
unesp.author.lattes5481756528299469[5]
unesp.author.orcid0000-0003-1452-5708[6]
unesp.author.orcid0000-0003-2938-010X[5]

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Botucatu - CEVAP - Centro de Estudos de Venenos e Animais Peçonhentos