In vivo photodynamic inactivation of Candida albicans using chloro-aluminum phthalocyanine

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dc.contributor.authorCarmello, J. C. [UNESP]
dc.contributor.authorAlves, F. [UNESP]
dc.contributor.authorRibeiro, A. P.D.
dc.contributor.authorBasso, F. G. [UNESP]
dc.contributor.authorde Souza Costa, C. A. [UNESP]
dc.contributor.authorTedesco, A. C.
dc.contributor.authorPrimo, F. L. [UNESP]
dc.contributor.authorMima, E. G. [UNESP]
dc.contributor.authorPavarina, A. C. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUnB - University of Brasília
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2018-12-11T17:03:21Z
dc.date.available2018-12-11T17:03:21Z
dc.date.issued2016-07-01
dc.description.abstractThis study evaluated the photoinactivation of Candida albicans in a murine model of oral candidiasis using chloro-aluminum phthalocyanine (ClAlP) encapsulated in cationic nanoemulsions (NE) and chloro-aluminum phthalocyanine (ClAlP) diluted in DMSO (DMSO) as photosensitizer (PS). Seventy-five 6-week-old female Swiss mice were immunosuppressed and inoculated with C. albicans to induce oral candidiasis. PDT was performed on the tongue by the application of the photosensitizers and LED light (100 J cm−2–660 nm). Twenty-four hours and 7 days after treatments, microbiological evaluation was carried out by recovering C. albicans from the tongue of animals (CFU ml−1). Then, mice were sacrificed and the tongues were surgically removed for histological and biomolecular analysis of pro- and anti-inflammatory cytokines. Data were analyzed by ANOVA followed by Tukey's post hoc test. ClAlP-NE-mediated PDT reduced 2.26 log10 of C. albicans recovered from the tongue when compared with the control group (P−L−) (P < 0.05). PDT did not promote adverse effects on the tongue tissue. Seven days after treatment, all animals were completely healthy. In summary, PDT mediated by chloro-aluminum phthalocyanine entrapped in cationic nanoemulsions was effective in reducing C. albicans recovered from the oral lesions of immunocompromised mice.en
dc.description.affiliationDepartment of Dental Materials and Prosthodontics Araraquara Dental School UNESP- Univ Estadual Paulista
dc.description.affiliationDepartment of Dentistry School of Health Science UnB - University of Brasília
dc.description.affiliationDepartment of Physiology and Pathology Araraquara Dental School UNESP- Univ Estadual Paulista
dc.description.affiliationCenter of Nanotechnology and Tissue Engineers Photobiology and Photomedicine Research Group FFCLRP—São Paulo University
dc.description.affiliationDepartment of Bioprocess and Biotechnology School of Pharmaceutical Sciences UNESP- Univ Estadual Paulista
dc.description.affiliationUnespDepartment of Dental Materials and Prosthodontics Araraquara Dental School UNESP- Univ Estadual Paulista
dc.description.affiliationUnespDepartment of Physiology and Pathology Araraquara Dental School UNESP- Univ Estadual Paulista
dc.description.affiliationUnespDepartment of Bioprocess and Biotechnology School of Pharmaceutical Sciences UNESP- Univ Estadual Paulista
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2012/17468-2
dc.format.extent415-422
dc.identifierhttp://dx.doi.org/10.1111/odi.12466
dc.identifier.citationOral Diseases, v. 22, n. 5, p. 415-422, 2016.
dc.identifier.doi10.1111/odi.12466
dc.identifier.issn1601-0825
dc.identifier.issn1354-523X
dc.identifier.scopus2-s2.0-84973596210
dc.identifier.urihttp://hdl.handle.net/11449/173065
dc.language.isoeng
dc.relation.ispartofOral Diseases
dc.relation.ispartofsjr0,798
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCandida albicans
dc.subjectchloro-aluminum phthalocyanine
dc.subjectmurine model
dc.titleIn vivo photodynamic inactivation of Candida albicans using chloro-aluminum phthalocyanineen
dc.typeArtigo
unesp.author.lattes8867670539105403[9]
unesp.author.lattes4517484241515548[5]
unesp.author.orcid0000-0002-9231-1994[9]
unesp.author.orcid0000-0002-7455-6867[5]

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