Influência das medidas de carcaça avaliadas por ultrassonografia em doadoras da raça senepol na produção in vitro de embriões.
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2023-07-31
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Universidade Estadual Paulista (Unesp)
Resumo
A técnica da ultrassonografia de carcaça possibilita avaliar a área de olho de lombo corrigida para 100 (AOL 100), espessura de gordura subcutânea corrigida para 100 (EGS 100), escore de gordura subcutânea na garupa (EGSG) e escore de marmoreio (EM). Atualmente tais avaliações são realizadas em doadoras de oócitos, em programas de produção in vitro de embriões, apenas como medidas potenciais de alta herdabilidade para incrementar a quantidade e qualidade da carne produzida nas progênies. Em relação aos aspectos produtivos de tais doadoras, almeja-se selecionar fêmeas que contemplem AOL > 80 cm2, EGS > 5 mm e EM > 5, porém tais mensurações nunca foram correlacionadas com a produção de embriões in vitro (PIVE). A hipótese é que as medidas de AOL 100, EGS 100, EGSG e EM em vacas e novilhas doadoras de oócitos da raça Senepol, exercem efeitos no potencial de produção de oócitos e embriões durante a PIVE. Objetivou-se avaliar, utilizando um banco de dados, a influência das medidas obtidas por ultrassonografia de carcaça sobre a produção in vitro de embriões em doadoras da raça Senepol. As medidas de ultrassonografia de carcaça (AOL 100, EGS 100, EGSG e EM) f oram realizadas antes da primeira aspiração folicular, e as fêmeas foram submetidas a no mínimo três aspirações consecutivas, compondo o banco de dados 42 novilhas (< 3 anos de idade) que contabilizam um total de 253 aspirações (Banco de Dados 1) e 34 vacas (6 a 10 anos de idade) que contabilizam um total de 185 aspirações (Banco de Dados 2). Os oócitos aspirados foram submetidos a um protocolo convencional comercial de maturação in vitro, fertilização in vitro e o cultivo in vitro dos prováveis zigotos. Para cada categoria (novilha e vaca) e cada medida (AOL 100, EGS 100, EGSG e EM) a mediana foi estabelecida para a divisão dos grupos, onde valores abaixo da mediana constituíram o grupo baixo e acima da mediana o grupo alto. Para as novilhas, o modelo considerou o efeito fixo das classes de AOL 100 (Baixa < 16,04 e Alto > 16,04), EGS 100 (Baixa < 1,11 e Alta > 1,11), EGSG (Baixa < 7,57 e Alta > 7,57) e EM (Baixa < 3,44 e Alta > 3,44). Para as vacas, o modelo considerou o efeito fixo das classes de AOL 100 (Baixa < 13,86 e Alto > 13,86), EGS 100 (Baixa < 1,08 e Alta > 1,08), EGSG picanha (Baixa < 9,41 e Alta > 9,41) e EM (Baixa < 4,00 e Alta > 4,00). A comparação das médias entre alto e baixo para cada característica foi realizada por uma análise de variância pelo PROC MIXED do SAS e os coeficientes de correlação foram determinados por meio do PROC CORR. AOL 100, EGS 100 e EGSG, nas vacas e novilhas doadoras não exerceram efeito no potencial de produção de oócitos e embriões durante a PIVE. O EM exerceu efeito, onde novilhas com EM alto apresentaram menor número de oócitos inviáveis (6,7541 ± 0,3100 vs. 9,1756 ± 0,5349 respectivamente; P = 0,0515), maior taxa de clivagem (65,45% vs. 59,59% respectivamente; P = 0,0298) e maior taxa de embriões (20,38% vs. 13,54% respectivamente; P = 0,0011) e vacas com EM alto apresentaram maior número de embriões produzidos (7,0400 ± 0,7172 vs. 5,0000 ± 0,5540 respectivamente; P = 0,0060) e maior taxa de embriões (43,44% vs. 27,74% respectivamente; P = 0,0034). Concluímos que o EM exerce efeito na qualidade dos oócitos e em ambas as categorias exerce efeito na produção de embriões durante a PIVE em fêmeas da raça Senepol.
Carcass ultrasonography technique makes it possible to evaluate the loin eye area corrected for 100 (AOL 100), subcutaneous fat thickness corrected for 100 (SFT 100), rump subcutaneous fat score (RSFS) and marbling score (MS). Currently, such evaluations are carried out in oocyte donors, in in vitro embryo production programs, only as potential measures of high heritability to increase the quantity and quality of the meat produced in the progenies. Regarding the productive aspects of such donors, the aim is to select females that include AOL > 80 cm2, EGS > 5 mm and EM > 5, but such measurements in have never been correlated with in vitro embryo production (IVP). The hypothesis is that the AOL 100, EGS 100, EGSG and EM measurements in Senepol breed oocyte donor cows and heifers determine effects on the production potential of oocytes and embryos production during IVP. The aim was to evaluated, using a database, the influence of measurements obtained by carcass ultrasound on the in vitro production of embryos in Senepol breed donors. Carcass ultrasound measurements (AOL 100, SFT 100, RSFT and MS) were made before the first follicular aspiration, and the females were subjected to at least three consecutive aspirations, composing the database 42 heifers (< 3 years old) that account for a total of 253 aspirations (Database 1) and 34 cows (6 to 10 years old) that account for a total of 185 aspirations (Database 2). The aspirated oocytes were submitted to a conventional commercial protocol of in vitro maturation, in vitro fertilization and the in vitro culture of probable zygotes. For each category (heifer and cow) and each measure (AOL 100, EGS 100, EGS and EM) the median was established for the division of groups, where values below the nedian constituted the low group and above the median the high group. For heifers, the model considered the fixed effect of AOL 100 (Low < 16.04 and High > 16.06), SFT 100 (Low < 1.114 and High > 1.11), RSFS (Low < 7.57 and High > 7.57) and MS (Low < 3.44 and High > 3.44) classes. For cows, the model considered the fixed effect of AOL 100 (Low < 13.86 and High > 13.86), SFT 100 (Low < 1.08 and High > 1.08), RSFS (Low < 9.41 and High > 9.41) and MS (Low < 4.00 and High > 4.00) classes. Comparison of the averages between high and low for each characteristic was performed by an analysis of variance using PROC MIXED of SAS and the correlation coefficients were determined using PROC CORR. AOL 100, SFT 100 and RSFS in donor cows and heifers had to effect on the production potential of oocytes and embryos during IVP. The EM had an effect, where heifers with high MS compared to those with low MS had a lower number of unviable oocytes (6.7541 ± 0.3100 vs. 9.1756 ± 0.5349 respectively; P = 0.0515), higher cleavage rate (65.45% vs. 59.59% respectively; P = 0.0298) and higher embryo rate (20.38% vs. 13.54% respectively; P = 0.0011) and cows with high MS when compared to those with low MS had a higher number of embryos produced (7.0400 ± 0.7172 vs. 5.0000 ± 0,5540 respectively; P = 0.0060) and a higher rate of embryos (43.44% vs. 27.74% respectively; P = 0.0034). We conclude that MS has an effect on oocyte quality and in the both categories it has an effect on embryo production during IVP in females of the Senepol Breed
Carcass ultrasonography technique makes it possible to evaluate the loin eye area corrected for 100 (AOL 100), subcutaneous fat thickness corrected for 100 (SFT 100), rump subcutaneous fat score (RSFS) and marbling score (MS). Currently, such evaluations are carried out in oocyte donors, in in vitro embryo production programs, only as potential measures of high heritability to increase the quantity and quality of the meat produced in the progenies. Regarding the productive aspects of such donors, the aim is to select females that include AOL > 80 cm2, EGS > 5 mm and EM > 5, but such measurements in have never been correlated with in vitro embryo production (IVP). The hypothesis is that the AOL 100, EGS 100, EGSG and EM measurements in Senepol breed oocyte donor cows and heifers determine effects on the production potential of oocytes and embryos production during IVP. The aim was to evaluated, using a database, the influence of measurements obtained by carcass ultrasound on the in vitro production of embryos in Senepol breed donors. Carcass ultrasound measurements (AOL 100, SFT 100, RSFT and MS) were made before the first follicular aspiration, and the females were subjected to at least three consecutive aspirations, composing the database 42 heifers (< 3 years old) that account for a total of 253 aspirations (Database 1) and 34 cows (6 to 10 years old) that account for a total of 185 aspirations (Database 2). The aspirated oocytes were submitted to a conventional commercial protocol of in vitro maturation, in vitro fertilization and the in vitro culture of probable zygotes. For each category (heifer and cow) and each measure (AOL 100, EGS 100, EGS and EM) the median was established for the division of groups, where values below the nedian constituted the low group and above the median the high group. For heifers, the model considered the fixed effect of AOL 100 (Low < 16.04 and High > 16.06), SFT 100 (Low < 1.114 and High > 1.11), RSFS (Low < 7.57 and High > 7.57) and MS (Low < 3.44 and High > 3.44) classes. For cows, the model considered the fixed effect of AOL 100 (Low < 13.86 and High > 13.86), SFT 100 (Low < 1.08 and High > 1.08), RSFS (Low < 9.41 and High > 9.41) and MS (Low < 4.00 and High > 4.00) classes. Comparison of the averages between high and low for each characteristic was performed by an analysis of variance using PROC MIXED of SAS and the correlation coefficients were determined using PROC CORR. AOL 100, SFT 100 and RSFS in donor cows and heifers had to effect on the production potential of oocytes and embryos during IVP. The EM had an effect, where heifers with high MS compared to those with low MS had a lower number of unviable oocytes (6.7541 ± 0.3100 vs. 9.1756 ± 0.5349 respectively; P = 0.0515), higher cleavage rate (65.45% vs. 59.59% respectively; P = 0.0298) and higher embryo rate (20.38% vs. 13.54% respectively; P = 0.0011) and cows with high MS when compared to those with low MS had a higher number of embryos produced (7.0400 ± 0.7172 vs. 5.0000 ± 0,5540 respectively; P = 0.0060) and a higher rate of embryos (43.44% vs. 27.74% respectively; P = 0.0034). We conclude that MS has an effect on oocyte quality and in the both categories it has an effect on embryo production during IVP in females of the Senepol Breed
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Palavras-chave
Carcaças, Nutrição animal, Oócitos, Embriões, Bovinos, Carcasses, Animals Nutrition, Oocytes, Embryos, Cattle, Bovine
Como citar
ZUCOLOTO, W. T. Influência das medidas de carcaça avaliadas por ultrassonografia em doadoras da raça Senepol na produção in vitro de embriões. 2023. 67 f. Dissertação (Mestrado em Ciência e Tecnologia Animal) - Universidade Estadual Paulista (UNESP), Dracena, 2023.