Chain cleavage of bioinspired bacterial membranes photoinduced by eosin decyl ester

Página do item simplificado
dc.contributor.authorMoreira, Lucas Gontijo
dc.contributor.authorAlmeida Junior, Alexandre Mendes
dc.contributor.authorCamacho, Sabrina Alessio [UNESP]
dc.contributor.authorEstevão, Bianca Martins
dc.contributor.authorOliveira Junior, Osvaldo Novais
dc.contributor.authorAoki, Pedro Henrique Benites [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2021-03-12T22:35:17Z
dc.date.available2021-03-12T22:35:17Z
dc.date.issued2020-07-16
dc.description.abstractPhotodynamic therapy (PDT) is promising for bacterial inactivation since cellular internalization of photosensitizers (PS) is not crucial for the treatment effectiveness. Photoinduced damage in the lipid envelope may already induce microbial inactivation, which requires PS capable of easily penetrating into the membrane. Herein, we report on the insertion of the PS eosin decyl ester (EosDec) into Langmuir films of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dioleoyl-sn-glycero-3-phospho-(1′-rac-glycerol) (DOPG), and cardiolipin (CLP) used as mimetic systems of bacterial membranes. Surface pressure isotherms and polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS) indicated that the hydrophobic nature of EosDec favored deeper penetration in all the phospholipid monolayers. The incorporation of EosDec led to monolayer expansion, especially in the anionic DOPG and CLP owing to repulsive electrostatic interactions, and induced disorder in the lipid chains. Light irradiation of DOPE, DOPG, and CLP monolayers containing EosDec increased the rate of material loss to the subphase, which is attributed to cleavage of lipid chains triggered by contact-dependent reactions between excited states of EosDec and lipid unsaturations. The latter is key for membrane permeabilization and efficiency in microbial inactivation.en
dc.description.affiliationFaculdade de Ciências e Letras de Assis (UNESP)
dc.description.affiliationInstituto de Física de São Carlos (USP)
dc.description.affiliationUniversidade Estadual de Maringá (UEM)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2013/14262-7
dc.description.sponsorshipIdFAPESP: 2018/13021-0
dc.description.sponsorshipIdFAPESP: 2018/16713-0
dc.description.sponsorshipIdFAPESP: 2018/14692-5
dc.description.sponsorshipIdFAPESP: 2018/22214-6
dc.description.sponsorshipIdCNPq: 403713/2016-1
dc.description.versionPostprintpt
dc.identifier.doi10.1021/acs.langmuir.0c01600
dc.identifier.issn1520-5827
dc.identifier.lattes0468519295693199
dc.identifier.lattes0543007566557863
dc.identifier.lattes5894291702537833
dc.identifier.lattes9230208959374582
dc.identifier.lattes8582867831317500
dc.identifier.lattes7384168674539702
dc.identifier.orcid0000-0002-2784-687X
dc.identifier.orcid0000-0002-7063-0812
dc.identifier.orcid0000-0002-6590-759X
dc.identifier.orcid0000-0001-7913-6979
dc.identifier.orcid0000-0002-5399-5860
dc.identifier.orcid0000-0003-4701-6408
dc.identifier.urihttp://hdl.handle.net/11449/204085
dc.language.isoeng
dc.publisherACS Publications
dc.relation.ispartofLangmuirpt
dc.rights.accessRightsAcesso aberto
dc.subjectMembranas (Biologia)pt
dc.subjectAgentes fotossensibilizantespt
dc.subjectTerapia fotodinâmicapt
dc.subjectInativação bacterianapt
dc.titleChain cleavage of bioinspired bacterial membranes photoinduced by eosin decyl esteren
dc.typeArtigo
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Letras, Assispt

Arquivos

Pacote Original
Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
moreira_lg_postprint_assis_chain.pdf
Tamanho:
1.12 MB
Formato:
Adobe Portable Document Format
Baixar
Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
2.25 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Biotecnologia - FCLAS