Cytogenetics characterization of Crenuchus spilurus (Günther, 1863): a remarkable low diploid value within family Crenuchidae (Characiformes)

Página do item simplificado
dc.contributor.authorPazian, Marlon F. [UNESP]
dc.contributor.authorOliveira, Claudio [UNESP]
dc.contributor.authorForesti, Fausto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2018-12-11T17:36:24Z
dc.date.available2018-12-11T17:36:24Z
dc.date.issued2018-01-01
dc.description.abstractConventional and molecular cytogenetic analyses were performed in specimens of the Neotropical Crenuchus spilurus freshwater fish species from a single location (Caeté River, Brazil). All specimens presented diploid values of 2n = 38 chromosomes (12 m + 4sm + 2st + 20a), the lowest reported for family Crenuchidae up to now. A single pair of nucleolar organizing regions (NORs) was detected in the subtelocentric chromosome pair no. 9 by silver-staining and fluorescence in situ hybridization (FISH) with 18S rDNA sequence-specific probe. Two pairs of 5S rRNA gene clusters were found either interstitial or terminally located in the long arms of the acrocentric chromosome pairs nos. 10 and 13. Heterochromatic regions were clearly observed in the short arms of the NOR-bearing chromosome pair and weakly-positive to the pericentromeric regions of most acrocentric chromosomes. Additionally, no sex chromosomes were identified in the surveyed specimens. Crenuchidae have signals of several mechanisms involved in karyotype diversification within this family: differential location of heterochromatin-rich regions, multiplication, and translocation of rDNA clusters, presence/absence of sex chromosomes, macrostructural changes in morphology and number of chromosomes. This variety of karyotype patterns reveals the importance of widening cytogenetic studies to more taxa for better know the chromosomal evolution occurred in this group.en
dc.description.affiliationLaboratório de Biologia e Genética de Peixes Instituto de Biociências Universidade Estadual Paulista (UNESP)
dc.description.affiliationUnespLaboratório de Biologia e Genética de Peixes Instituto de Biociências Universidade Estadual Paulista (UNESP)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 2009/50952-2
dc.format.extent77-81
dc.identifierhttp://dx.doi.org/10.2478/s11756-018-0006-9
dc.identifier.citationBiologia (Poland), v. 73, n. 1, p. 77-81, 2018.
dc.identifier.doi10.2478/s11756-018-0006-9
dc.identifier.issn1336-9563
dc.identifier.issn0006-3088
dc.identifier.scopus2-s2.0-85044349245
dc.identifier.urihttp://hdl.handle.net/11449/179697
dc.language.isoeng
dc.relation.ispartofBiologia (Poland)
dc.relation.ispartofsjr0,299
dc.relation.ispartofsjr0,299
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subject18S and 5S rDNA
dc.subjectFISH
dc.subjectFreshwater fishes
dc.subjectNeotropical fishes
dc.titleCytogenetics characterization of Crenuchus spilurus (Günther, 1863): a remarkable low diploid value within family Crenuchidae (Characiformes)en
dc.typeArtigo
unesp.author.orcid0000-0003-3922-2551[1]

Arquivos

Coleções

Artigos - Morfologia - IBB