Strain differentiation of Trichophyton rubrum by randomly amplified polymorphic DNA and analysis of rDNA nontranscribed spacer

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dc.contributor.authorBaeza, L. C.
dc.contributor.authorMatsumoto, M. T.
dc.contributor.authorAlmeida, Ana Marisa Fusco [UNESP]
dc.contributor.authorMendes-Giannini, Maria José Soares [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:24:30Z
dc.date.available2014-05-20T13:24:30Z
dc.date.issued2006-04-01
dc.description.abstractTrichophyton rubrum is the most common pathogen causing dermatophytosis. Molecular strain-typing methods have recently been developed to tackle epidemiological questions and the problem of relapse following treatment. A total of 67 strains of T rubrum were screened for genetic variation by randomly amplified polymorphic DNA (RAPD) analysis, with two primers, 5'-d[GGTGCGGGAA]-3' and 5'-d[CCCGTCAGCA]-3', as well as by subrepeat element analysis of the nontranscribed spacer of rDNA, using the repetitive subelements TRS-1 and TRS-2. A total of 12 individual patterns were recognized with the first primer and 11 with the second. Phylogenetic analysis of the RAPID products showed a high degree of similarity (> 90 %) among the epidemiologically related clinical isolates, while the other strains possessed 60% similarity. Specific amplification of TRS-1 produced three strain-characteristic banding patterns (PCR types); simple patterns representing one copy of TRS-1 and two copies of TRS-2 accounted for around 85 % of all isolates. It is concluded that molecular analysis has important implications for epidemiological studies, and RAPID analysis is especially suitable for molecular typing in T. rubrum.en
dc.description.affiliationUNESP, Fac Ciências Farmaceut, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil
dc.description.affiliationUnespUNESP, Fac Ciências Farmaceut, Dept Anal Clin, BR-14801902 Araraquara, SP, Brazil
dc.format.extent429-436
dc.identifierhttp://dx.doi.org/10.1099/jmm.0.46236-0
dc.identifier.citationJournal of Medical Microbiology. Reading: Soc General Microbiology, v. 55, n. 4, p. 429-436, 2006.
dc.identifier.doi10.1099/jmm.0.46236-0
dc.identifier.issn0022-2615
dc.identifier.orcid0000-0002-8059-0826
dc.identifier.urihttp://hdl.handle.net/11449/7621
dc.identifier.wosWOS:000236489800011
dc.language.isoeng
dc.publisherSoc General Microbiology
dc.relation.ispartofJournal of Medical Microbiology
dc.relation.ispartofjcr2.112
dc.relation.ispartofsjr0,914
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleStrain differentiation of Trichophyton rubrum by randomly amplified polymorphic DNA and analysis of rDNA nontranscribed spaceren
dc.typeArtigo
dcterms.licensehttp://jmm.sgmjournals.org/site/misc/reprints.xhtml
dcterms.rightsHolderSoc General Microbiology
unesp.author.lattes3716273524139678[3]
unesp.author.orcid0000-0002-8059-0826[4]
unesp.author.orcid0000-0002-2115-8988[3]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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Artigos - Análises Clínicas - FCFAR