Genotoxicity Evaluation in Severe or Mild Diabetic Pregnancy in Laboratory Animals

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dc.contributor.authorLima, P. H. O. [UNESP]
dc.contributor.authorSinzato, Y. K. [UNESP]
dc.contributor.authorGelaleti, R. B. [UNESP]
dc.contributor.authorCalderon, Iracema de Mattos Paranhos [UNESP]
dc.contributor.authorRudge, Marilza Vieira Cunha [UNESP]
dc.contributor.authorDamasceno, Débora Cristina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:35:29Z
dc.date.available2014-05-20T13:35:29Z
dc.date.issued2012-05-01
dc.description.abstractThis study aimed to evaluate the genotoxicity (DNA damage levels) in lymphocyte samples from pregnant Wistar rats with severe or mild diabetes and in whole blood samples from their newborns. Wistar female rats (1 and 90 days of age) and male rats (approximately 90 days of age) were used. The experiment consisted of 2 experimental groups (n = 8 animals/group): 1) rats with severe diabetes, 2) rats with mild diabetes. For mild diabetes induction, the rats received streptozotocin (STZ) subcutaneously (100 mg/kg body weight) at day of birth, and those showing glycemia from 120 to 300 mg/dL in their adult life were included. For induction of severe diabetes, adult rats received 40 mg/kg STZ (intravenous route), and those showing glycemia >300 mg/dL were included. At day 21 of pregnancy, the, rats were anesthetized and euthanized for removal of maternal and fetal blood samples for determination of the oxidative DNA damage by applying Endo III and Fpg using the comet assay. Thus, the rats with mild diabetes and their offspring showed higher Fpg-sensitive sites, reflecting the damage resulting from hyperglycemia. The rats with severe diabetes and their offspring showed higher oxidative DNA damage detected by Fpg and Endo III-sensitive sites, showing general repercussions related to diabetes. The enzymatic treatment for DNA damage evidenced that the maternal repercussions of diabetes are associated with oxidative DNA damage of their newborn, which was not reflected using only the analysis of DNA damage free of the enzymes.en
dc.description.affiliationUniv Estadual Paulista Unesp, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista Unesp, Botucatu Med Sch, Dept Gynecol & Obstet, Lab Expt Res Gynecol & Obstet, Botucatu, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 06/06056-4
dc.format.extent303-307
dc.identifierhttp://dx.doi.org/10.1055/s-0031-1299766
dc.identifier.citationExperimental and Clinical Endocrinology & Diabetes. Stuttgart: Johann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh, v. 120, n. 5, p. 303-307, 2012.
dc.identifier.doi10.1055/s-0031-1299766
dc.identifier.issn0947-7349
dc.identifier.lattes0679387622604743
dc.identifier.lattes6758680388835078
dc.identifier.orcid0000-0002-9227-832X
dc.identifier.urihttp://hdl.handle.net/11449/12216
dc.identifier.wosWOS:000305862200011
dc.language.isoeng
dc.publisherJohann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh
dc.relation.ispartofExperimental and Clinical Endocrinology & Diabetes
dc.relation.ispartofjcr1.623
dc.relation.ispartofsjr0,695
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdiabetesen
dc.subjectDNA damageen
dc.subjectoffspringen
dc.subjectcomet assayen
dc.subjectraten
dc.subjectoxidative stressen
dc.titleGenotoxicity Evaluation in Severe or Mild Diabetic Pregnancy in Laboratory Animalsen
dc.typeArtigo
dcterms.licensehttp://www.thieme.de/fz/_pdf/thieme_ir_policy_english.pdf
dcterms.rightsHolderJohann Ambrosius Barth Verlag Medizinverlage Heidelberg Gmbh
unesp.author.lattes0679387622604743
unesp.author.lattes6758680388835078
unesp.author.orcid0000-0002-6098-9899[3]
unesp.author.orcid0000-0002-7003-9643[6]
unesp.author.orcid0000-0003-4761-4336[4]
unesp.author.orcid0000-0002-9227-832X[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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