Peri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifers

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dc.contributor.authorSilva, Felipe A C C
dc.contributor.authorda Silva, Gabriela F.
dc.contributor.authorVieira, Bruna S.
dc.contributor.authorNeto, Adomar L.
dc.contributor.authorRocha, Cecilia C.
dc.contributor.authorLo Turco, Edson G.
dc.contributor.authorNogueira, Guilherme P. [UNESP]
dc.contributor.authorPugliesi, Guilherme
dc.contributor.authorBinelli, Mario
dc.contributor.institutionFL
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:46:14Z
dc.date.available2022-04-29T08:46:14Z
dc.date.issued2021-11-15
dc.description.abstractIn cattle, uterine luminal fluid (ULF) is the main source of molecules that support embryo development and survival during the peri-implantation period. Our overarching hypothesis is that peri-estrus changes in uterine function, including ULF accumulation and absorption, are uneven among individuals, and affect ULF composition and fertility. Our objectives were (1) to characterize temporal and spatial changes in ULF volume, endometrial and luteal blood perfusion, endometrial and luteal size, and circulating progesterone concentrations during the peri-estrus period in beef heifers and (2) to associate such changes with the metabolite composition in the ULF, 4 days after estrus (d 0). Fourteen Bos indicus heifer that presented a PGF2α responsive CL received 500 μg PGF2α analog i.m. and were examined daily by rectal B-mode and pulse-wave color-Doppler ultrasonography until the fifth day after estrus (d 5). The composition of the ULF was analyzed by targeted mass spectrometry on d 4. Multivariate analyses clustered heifers according to ovarian, uterine, and hormonal variables in clusters A (n = 5) and B (n = 8 heifers). Concentrations of Pro, Ala, Leu, Gly, Val, Lys, Ile, Phe, Asp, Orn, Tyr, Arg, Trp, Suc, Cit, ADMA, the sum of essential Amino Acids (AA), sum of nonessential AA, sum of aromatic AA, and total AA were greater in cluster A (FDR ≤ 0.05). ULF volume dynamics and uterine, ovarian, and hormonal variables during the peri-estrus period presented a concerted variation among heifers within clusters, which was associated with the ULF composition 4 days after estrus.en
dc.description.affiliationDepartment of Animal Sciences University of Florida FL
dc.description.affiliationDepartment of Animal Reproduction School of Veterinary Medicine and Animal Sciences University of São Paulo
dc.description.affiliationDepartment of Surgery Division of Urology Federal University of São Paulo
dc.description.affiliationSchool of Veterinary Medicine São Paulo State University
dc.description.affiliationUnespSchool of Veterinary Medicine São Paulo State University
dc.format.extent1140-1153
dc.identifierhttp://dx.doi.org/10.1093/biolre/ioab149
dc.identifier.citationBiology of reproduction, v. 105, n. 5, p. 1140-1153, 2021.
dc.identifier.doi10.1093/biolre/ioab149
dc.identifier.issn1529-7268
dc.identifier.scopus2-s2.0-85121967258
dc.identifier.urihttp://hdl.handle.net/11449/231579
dc.language.isoeng
dc.relation.ispartofBiology of reproduction
dc.sourceScopus
dc.subjectbeef heifers
dc.subjectperi-estrus period
dc.subjectuterine luminal fluid metabolome
dc.titlePeri-estrus ovarian, uterine, and hormonal variables determine the uterine luminal fluid metabolome in beef heifersen
dc.typeArtigo

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