Formulation of Pseudomonas chlororaphis strains for improved shelf life

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dc.contributor.authorCorrea, E. B. [UNESP]
dc.contributor.authorSutton, J. C.
dc.contributor.authorBettiol, Wagner [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv State Paraiba
dc.contributor.institutionUniv Guelph
dc.contributor.institutionEmpresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.date.accessioned2015-03-18T15:53:20Z
dc.date.available2015-03-18T15:53:20Z
dc.date.issued2015-01-01
dc.description.abstractFormulations of Pseudomonas strains with long-term shelf life are needed for commercial use in biological disease control and growth promotion in crops. In the present work Pseudomonas chlororaphis (Pc) 63-28 formulated with coconut fiber [moisture content (MC) of 80%], talc (MC 8%) or peat (MC 40%), with or without the addition of carboxymethylcellulose or xanthan gum, and formulations of Pc 63-28 and P. chlororaphis TX-1 in coconut fiber with water contents (v:v) of 75%, 45%, and 25%, were evaluated in terms of shelf life and cell viability. The shelf life of Pc 63-28 was longer when formulated in coconut fibre with a MC was 80% than in the other formulations and longer at 3 +/- 1 degrees C compared to 22 +/- 1 degrees C. Densities of viable Pc 63-28 cells in coconut fiber stored at 3 +/- 1 degrees C did not decline significantly during 224 days when the MC was 80% and within 120 days at 75% MC. Densities of Pc TX-1 in coconut fiber of 75% MC did not decline within 60 days at 3 +/- 1 degrees C. P. chlororaphis 63-28 survived longer in deionized water and buffer than in canola oil. Cells of Pc 63-28 cells formulated in coconut fibre of 80% MC after storage for 140 days at 3 +/- 1 degrees C in coconut fiber improved hydroponic growth of hydroponic lettuce and better than cells freshly recovered from culture. We conclude that coconut fiber is a carrier of superior performance in maintaining shelf life of Pseudomonas strains. The observed shelf life would be sufficient for practical use of Pseudomonas strains as tools for disease control and growth promotion in crops. (C) 2014 Elsevier Inc. All rights reserved.en
dc.description.affiliationSao Paulo State Univ Julio Mesquita Filho, Dept Plant Protect, BR-18610307 Botucatu, SP, Brazil
dc.description.affiliationUniv State Paraiba, BR-58117000 Lagoa Seca, PB, Brazil
dc.description.affiliationUniv Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada
dc.description.affiliationEmbrapa Environm, BR-13820000 Jaguariuna, SP, Brazil
dc.description.affiliationUnespSao Paulo State Univ Julio Mesquita Filho, Dept Plant Protect, BR-18610307 Botucatu, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent50-55
dc.identifierhttp://dx.doi.org/10.1016/j.biocontrol.2014.09.009
dc.identifier.citationBiological Control. San Diego: Academic Press Inc Elsevier Science, v. 80, p. 50-55, 2015.
dc.identifier.doi10.1016/j.biocontrol.2014.09.009
dc.identifier.issn1049-9644
dc.identifier.urihttp://hdl.handle.net/11449/116452
dc.identifier.wosWOS:000345586700007
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofBiological Control
dc.relation.ispartofjcr2.112
dc.relation.ispartofsjr0,950
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBioagentsen
dc.subjectCoconut fiberen
dc.subjectLactuca sativaen
dc.subjectBacterial formulationen
dc.titleFormulation of Pseudomonas chlororaphis strains for improved shelf lifeen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Agronômicas, Botucatupt

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