Effects of Qualea grandiflora Extract on the Expression of MMP-14 and HIF-1α in Cultured Fibroblasts and Preosteoblasts

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dc.contributor.authorde Oliveira, Gabriela Silva Neubern
dc.contributor.authorTokuhara, Cintia Kazuko
dc.contributor.authorde Oliveira, Flávia Amadeu
dc.contributor.authorGraeff, Márcia Sirlene Zardin
dc.contributor.authorKhan, Zohaib Nisar
dc.contributor.authorSaldanha, Luiz Leonardo [UNESP]
dc.contributor.authorDokkedal, Anne Lígia [UNESP]
dc.contributor.authorde Oliveira, Rodrigo Cardoso
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-29T08:39:50Z
dc.date.available2022-04-29T08:39:50Z
dc.date.issued2021-01-01
dc.description.abstractBackground: Qualea grandiflora (QG) (Vochysiaceae), also known as “pau-ferro”, “pau-terra” or “pau-de-tucano”, is a very common deciduous tree in the Brazilian Cerrado used in traditional medicine to treat inflammations, ulcers, diarrhea, and infections. There are reports in the scientific literature that demonstrate the medicinal effects of the bark and leaf of the QG. However, studies involving this plant are rather imited. Aim of the study: To perform the phytochemical analysis of the QG hydroalcoholic extract (HAE) of leaves, and to investigate it effects on fibroblast and preosteoblasts. Methods: Phytochemical analysis was done by HPLC-DAD. Murine NIH/3T3 fibroblasts and MC3T3-E1 preosteoblasts cell lines (ATCC) were used for the experiments. Cell viability was assessed by the MTT colorimetric assay and the expression of MMP-14 and HIF-1a by immunofluorescence. Results and conclusion: The following compounds were identified by HPLC-DAD, such as quinic acid, ethyl galate, ellagic acid derivatives as O-methylellagic acid O-galloyl, O-methylellagic acid O-deoxyhexoside, galloyl derivatives, flavonol glycoside as kaempferol-O-deoxyhexoside, quercetin-O-deoxyhexoside, myricetin-O-deoxyhexoside and the pentacyclic triterpene arjunglucoside. Cell viability results demonstrated no cytotoxic effects in the studied concentrations. We found in QG HAE some compounds with therapeutic properties that can increase the expression of MMP-14 and HIF-1α, in fibroblasts and preosteoblasts. These data suggest that QG HAE has an action on these two molecules widely involved in physiological conditions, such as collagen remodeling, bone development and growth and pathological processes as HIF signaling in cancer metastasis.en
dc.description.affiliationUniversity of São Paulo Bauru School of Dentistry Department of Biological Sciences
dc.description.affiliationSão Paulo State University Faculty of Sciences Department of Biological Sciences. c[Bauru
dc.description.affiliationUnespSão Paulo State University Faculty of Sciences Department of Biological Sciences. c[Bauru
dc.identifierhttp://dx.doi.org/10.1590/1678-4324-2021200714
dc.identifier.citationBrazilian Archives of Biology and Technology, v. 64.
dc.identifier.doi10.1590/1678-4324-2021200714
dc.identifier.issn1678-4324
dc.identifier.issn1516-8913
dc.identifier.scopus2-s2.0-85124833630
dc.identifier.urihttp://hdl.handle.net/11449/230414
dc.language.isoeng
dc.relation.ispartofBrazilian Archives of Biology and Technology
dc.sourceScopus
dc.subjectHypoxia-inducible factor 1-alpha
dc.subjectMatrix Metalloproteinase-14
dc.subjectMedicinal plant
dc.subjectQualea grandiflora
dc.subjectVochysiaceae
dc.titleEffects of Qualea grandiflora Extract on the Expression of MMP-14 and HIF-1α in Cultured Fibroblasts and Preosteoblastsen
dc.typeArtigo
unesp.author.orcid0000-0003-0037-5665[1]
unesp.author.orcid0000-0002-1852-5986[2]
unesp.author.orcid0000-0003-3063-6694[3]
unesp.author.orcid0000-0002-6854-7913[4]
unesp.author.orcid0000-0001-9233-8639[5]
unesp.author.orcid0000-0003-2454-067X[6]
unesp.author.orcid0000-0002-0213-8551[7]
unesp.author.orcid0000-0003-3070-5960[8]
unesp.departmentCiências Biológicas - FCpt

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