Evaluation of estrogen receptor alpha and beta and progesterone receptor expression and correlation with clinicopathologic factors and proliferative marker Ki-67 in breast cancers

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dc.contributor.authorRosa, Fabiola E. [UNESP]
dc.contributor.authorCaldeira, Jose R. F. [UNESP]
dc.contributor.authorFelipes, Joice
dc.contributor.authorBertonha, Fernanda B. [UNESP]
dc.contributor.authorQuevedo, Francisco C.
dc.contributor.authorDomingues, Maria Aparecida Custódio [UNESP]
dc.contributor.authorMoraes, Francisco A.
dc.contributor.authorRogatto, Silvia Regina [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionAmaral Carvalho Hosp
dc.contributor.institutionUniversidade Estadual de Maringá (UEM)
dc.date.accessioned2014-05-20T13:37:24Z
dc.date.available2014-05-20T13:37:24Z
dc.date.issued2008-05-01
dc.description.abstractTo elucidate the molecular profile of hormonal steroid receptor status, we analyzed ER-alpha, ER-beta, and PGR mRNA and protein expression in 80 breast carcinomas using reverse transcriptase polymerase chain reaction (RT-PCR), quantitative RT-PCR, and immunohistochemical analysis. Qualitative analysis revealed positive expression of ER-alpha, ER-beta, and PGR mRNA in 48%, 59%, and 48% of the breast carcinomas, respectively. ER-alpha, ER-beta, and PGR transcript overexpression was observed in 51%, 0%, and 12% of the cases, respectively, whereas moderate or strong protein expression was detected in 68%, 78%, and 49% of the cases, respectively. Tumor grade was negatively correlated with transcript and protein levels of ER-alpha (P = .0169 and P = .0006, respectively) and PGR (P = .0034 and P = .0005, respectively). Similarly, proliferative index Ki-67 was negatively associated with transcript and protein levels of ER-alpha (P = .0006 and P < .0001, respectively) and PGR (P = .0258 and P =. 0005, respectively). These findings suggest that ER-alpha and PGR expression are associated with well-differentiated breast tumors and less directly related to cell proliferation. A significant statistical difference was observed between lymph node status and ER-beta protein expression (P = .0208). In ER-alpha-negative tumors, we detected a correlation between ER-beta protein expression and high levels of Ki-67. These data suggest that ER-beta could be a prognostic marker in human breast cancer. (C) 2008 Elsevier B.V. All rights reserved.en
dc.description.affiliationSão Paulo State Univ, UNESP, Fac Med, Dept Urol,Neogene Lab, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Dept Genet, Inst Biosci, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationAmaral Carvalho Hosp, Dept Senol, São Paulo, Brazil
dc.description.affiliationUniversidade Estadual de Maringá (UEM), Dept Biol Celular & Genet, Maringa, Parana, Brazil
dc.description.affiliationAmaral Carvalho Hosp, Dept Pathol, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, UNESP, Fac Med, Dept Pathol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Fac Med, Dept Urol,Neogene Lab, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Dept Genet, Inst Biosci, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUnespSão Paulo State Univ, UNESP, Fac Med, Dept Pathol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent720-730
dc.identifierhttp://dx.doi.org/10.1016/j.humpath.2007.09.019
dc.identifier.citationHuman Pathology. Philadelphia: W B Saunders Co-elsevier Inc, v. 39, n. 5, p. 720-730, 2008.
dc.identifier.doi10.1016/j.humpath.2007.09.019
dc.identifier.issn0046-8177
dc.identifier.lattes2259986546265579
dc.identifier.lattes0585723113037140
dc.identifier.urihttp://hdl.handle.net/11449/12935
dc.identifier.wosWOS:000255730900011
dc.language.isoeng
dc.publisherW B Saunders Co-elsevier Inc
dc.relation.ispartofHuman Pathology
dc.relation.ispartofjcr3.125
dc.relation.ispartofsjr1,304
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbreast canceren
dc.subjectestrogen receptoren
dc.subjectprogesterone receptoren
dc.subjectmRNA expressionen
dc.subjectimmunohistochemistryen
dc.titleEvaluation of estrogen receptor alpha and beta and progesterone receptor expression and correlation with clinicopathologic factors and proliferative marker Ki-67 in breast cancersen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderW B Saunders Co-elsevier Inc
unesp.author.lattes2259986546265579
unesp.author.lattes0585723113037140
unesp.author.orcid0000-0002-3675-1362[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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