Cellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanisms

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dc.contributor.authorSakane, K. K.
dc.contributor.authorMonteiro, C. J.
dc.contributor.authorSilva, W.
dc.contributor.authorSilva, A. R.
dc.contributor.authorSantos, P. M.
dc.contributor.authorLima, K. F.
dc.contributor.authorMoraes, K. C. M. [UNESP]
dc.contributor.institutionUniv Vale Paraiba
dc.contributor.institutionUniversidade Federal de Ouro Preto (UFOP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-12-03T13:10:59Z
dc.date.available2014-12-03T13:10:59Z
dc.date.issued2014-01-01
dc.description.abstractCardiovascular disease is one of the leading causes of death worldwide, and evidence indicates a correlation between the inflammatory process and cardiac dysfunction. Selective inhibitors of cyclooxygenase-2 (COX-2) enzyme are not recommended for long-term use because of potentially severe side effects to the heart. Considering this and the frequent prescribing of commercial celecoxib, the present study analyzed cellular and molecular effects of 1 and 10 mu M celecoxib in a cell culture model. After a 24-h incubation, celecoxib reduced cell viability in a dose-dependent manner as also demonstrated in MTT assays. Furthermore, reverse transcription-polymerase chain reaction analysis showed that the drug modulated the expression level of genes related to death pathways, and Western blot analyses demonstrated a modulatory effect of the drug on COX-2 protein levels in cardiac cells. In addition, the results demonstrated a downregulation of prostaglandin E2 production by the cardiac cells incubated with celecoxib, in a dose-specific manner. These results are consistent with the decrease in cell viability and the presence of necrotic processes shown by Fourier transform infrared analysis, suggesting a direct correlation of prostanoids in cellular homeostasis and survival.en
dc.description.affiliationUniv Vale Paraiba, Inst Pesquisa & Desenvolvimento, Sao Jose Dos Campos, Brazil
dc.description.affiliationUniv Fed Ouro Preto, Nucleo Pesquisa Ciencias Biol, Ouro Preto, MG, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol, Inst Biociencias, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG)
dc.description.sponsorshipFundacao Valeparaibana de Ensino
dc.description.sponsorshipIdCNPq: 475586/2009-3
dc.description.sponsorshipIdCNPq: 506991/2010-5
dc.description.sponsorshipIdFAPEMIG: 02351-10
dc.format.extent50-59
dc.identifierhttp://dx.doi.org/10.1590/1414-431X20133028
dc.identifier.citationBrazilian Journal Of Medical And Biological Research. Sao Paulo: Assoc Bras Divulg Cientifica, v. 47, n. 1, p. 50-59, 2014.
dc.identifier.doi10.1590/1414-431X20133028
dc.identifier.fileS0100-879X2013005003028.pdf
dc.identifier.issn0100-879X
dc.identifier.scieloS0100-879X2013005003028
dc.identifier.urihttp://hdl.handle.net/11449/112703
dc.identifier.wosWOS:000331907500007
dc.language.isoeng
dc.publisherAssociação Brasileira de Divulgação Científica (ABRADIC)
dc.relation.ispartofBrazilian Journal of Medical and Biological Research
dc.relation.ispartofjcr1.492
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectCellular and molecular analysesen
dc.subjectCell deathen
dc.subjectCyclooxygenase-2 inhibitoren
dc.subjectH9c2 cardiac cell lineen
dc.titleCellular and molecular studies of the effects of a selective COX-2 inhibitor celecoxib in the cardiac cell line H9c2 and their correlation with death mechanismsen
dc.typeArtigo
dcterms.rightsHolderAssoc Bras Divulg Cientifica
unesp.author.orcid0000-0002-3789-6432[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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