Evaluation of Candida albicans adhesion and biofilm formation on a denture base acrylic resin containing silver nanoparticles

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dc.contributor.authorWady, A. F. [UNESP]
dc.contributor.authorMachado, Ana Lucia [UNESP]
dc.contributor.authorZucolotto, V. [UNESP]
dc.contributor.authorZamperini, C. A. [UNESP]
dc.contributor.authorBerni, E.
dc.contributor.authorVergani, Carlos Eduardo [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-20T13:46:33Z
dc.date.available2014-05-20T13:46:33Z
dc.date.issued2012-06-01
dc.description.abstractAim: This study firstly evaluated the activity of a silver nanoparticle (AgNPs) solution against Candida albicans and then the effect of incorporation of AgNPs into a denture base acrylic resin on the materials hydrophobicity, C.albicans adhesion and biofilm formation. Methods and Results: The AgNPs solution was synthesized by chemical reduction and characterized. Minimum inhibitory (MIC) and minimum fungicidal (MFC) concentrations for planktonic cells and sessile cells (MFCs) of the AgNPs solution against C.albicans were determined. Specimens (n = 360) of silver-incorporated acrylic resin at concentrations of 1000, 750, 500, 250 and 30 ppm were also prepared and stored in PBS for 0, 7, 90 and 180 days. Control was acrylic resin without AgNPs (0 ppm). After the storage periods, contact angles were measured and the specimens were used for C.albicans adherence (37 degrees C; 90 min; n = 9) and biofilm formation (37 degrees C; 48 h; n = 9) by XTT reduction assay. MIC, MFC and MFCs values were 3.98, 15.63 and 1000 ppm, respectively. Incorporation of AgNPs reduced the hydrophobicity of the resin. No effect on adherence and biofilm formation was observed. At 90 and 180 days of storage, there was significant increase in adherence and biofilm formation. Conclusions: Although the AgNPs solution had antifungal activity, no effect on C.albicans adherence and biofilm formation was observed after its incorporation into a denture base resin. Significance and Impact of the Study: The synthesized AgNPs solution is a promising antifungal agent, warranting investigations of more efficient methods of incorporation into denture base resins.en
dc.description.affiliationUniv Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, USP, Dept Phys & Informat, Phys Inst São Carlos, São Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, UNICAMP, Inst Chem, São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, UNESP, Araraquara Dent Sch, Dept Dent Mat & Prosthodont, São Paulo, Brazil
dc.description.affiliationUnespSão Paulo State Univ, USP, Dept Phys & Informat, Phys Inst São Carlos, São Paulo, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipIdFAPESP: 08/11700-5
dc.description.sponsorshipIdFAPESP: 08/07454-9
dc.format.extent1163-1172
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2672.2012.05293.x
dc.identifier.citationJournal of Applied Microbiology. Malden: Wiley-blackwell, v. 112, n. 6, p. 1163-1172, 2012.
dc.identifier.doi10.1111/j.1365-2672.2012.05293.x
dc.identifier.issn1364-5072
dc.identifier.lattes8000248781842587
dc.identifier.lattes3003130522427820
dc.identifier.orcid0000-0002-7375-4714
dc.identifier.urihttp://hdl.handle.net/11449/16486
dc.identifier.wosWOS:000303854700011
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofJournal of Applied Microbiology
dc.relation.ispartofjcr2.160
dc.relation.ispartofsjr0,795
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectbiofilmen
dc.subjectCandida albicansen
dc.subjectdenturesen
dc.subjectnanoparticlesen
dc.subjectstomatitisen
dc.titleEvaluation of Candida albicans adhesion and biofilm formation on a denture base acrylic resin containing silver nanoparticlesen
dc.typeArtigo
dcterms.licensehttp://www.nhm.ac.uk/hosted_sites/acarology/saas/saa/aim.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes8000248781842587[2]
unesp.author.lattes3003130522427820[6]
unesp.author.orcid0000-0002-7375-4714[6]
unesp.author.orcid0000-0002-2718-428X[2]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Odontologia, Araraquarapt

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