Immobilization of uricase in layer-by-layer films used in amperometric biosensors for uric acid

Página do item simplificado
dc.contributor.authorMoraes, Marli L.
dc.contributor.authorRodrigues Filho, Ubirajara P.
dc.contributor.authorOliveira, Osvaldo N.
dc.contributor.authorFerreira, Marystela [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:22:55Z
dc.date.available2014-05-20T13:22:55Z
dc.date.issued2007-11-01
dc.description.abstractThe layer-by-layer technique was exploited to immobilize the enzyme uricase onto indium tin oxide substrates coated with a layer of Prussian Blue. Uricase layers were alternated with either poly(ethylene imine) or poly(diallyidimethylammoniumchloride), and the resulting films were used as amperometric biosensors for uric acid. Biosensors with optimum perfomance had a limit of detection of 0.15 mu A mu mol 1(-1) cm(-2) with a linear response between 0.1 and 0.6 mu M of uric acid, which is sufficient for use in clinical tests. Bioactivity was preserved for weeks, and there was negligible influence from interferents, as detection was carried out at 0.0 V vs saturated calomel electrode.en
dc.description.affiliationUniv Estadual Paulista, Dept Quim Fis & Biol, BR-19060900 Presidente Prudente, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Quim Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
dc.description.affiliationUniv São Paulo, Inst Fis Sao Carlos, BR-13560970 Sao Carlos, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Quim Fis & Biol, BR-19060900 Presidente Prudente, SP, Brazil
dc.format.extent1489-1495
dc.identifierhttp://dx.doi.org/10.1007/s10008-007-0314-4
dc.identifier.citationJournal of Solid State Electrochemistry. New York: Springer, v. 11, n. 11, p. 1489-1495, 2007.
dc.identifier.doi10.1007/s10008-007-0314-4
dc.identifier.issn1432-8488
dc.identifier.urihttp://hdl.handle.net/11449/6805
dc.identifier.wosWOS:000251676900005
dc.language.isoeng
dc.publisherSpringer
dc.relation.ispartofJournal of Solid State Electrochemistry
dc.relation.ispartofjcr2.509
dc.relation.ispartofsjr0,661
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectlayer-by-layerpt
dc.subjectamperometric biosensorpt
dc.subjecturicasept
dc.subjectPrussian Bluept
dc.titleImmobilization of uricase in layer-by-layer films used in amperometric biosensors for uric aciden
dc.typeArtigo
dcterms.licensehttp://www.springer.com/open+access/authors+rights?SGWID=0-176704-12-683201-0
dcterms.rightsHolderSpringer
unesp.author.orcid0000-0002-5399-5860[3]
unesp.author.orcid0000-0003-0213-3686[1]
unesp.author.orcid0000-0001-6913-8540[2]
unesp.author.orcid0000-0002-9459-8167[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências e Tecnologia, Presidente Prudentept

Arquivos

Licença do Pacote
Agora exibindo 1 - 1 de 1
Nenhuma Miniatura disponível
Nome:
license.txt
Tamanho:
1.71 KB
Formato:
Item-specific license agreed upon to submission
Descrição:

Coleções

Artigos - Física, Química e Biologia - FCT