A study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of lead

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dc.contributor.authorTrama-Freitas, Bianca [UNESP]
dc.contributor.authorFreitas, Johnattan C.S. [UNESP]
dc.contributor.authorMartins, Rui C.
dc.contributor.authorGando-Ferreira, Licínio M.
dc.contributor.authorQuinta-Ferreira, Maria Emília
dc.contributor.authorQuinta-Ferreira, Rosa Maria
dc.contributor.authordo Carmo, Devaney R. [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversity of Coimbra
dc.date.accessioned2018-12-11T17:15:38Z
dc.date.available2018-12-11T17:15:38Z
dc.date.issued2017-12-20
dc.description.abstractLead is a heavy metal of high impact for the environment as well as for human health, being cause of several diseases. Considering the importance of obtaining an effective treatment for lead removal, a new hybrid material was developed for sorption of Pb2+ from aqueous solution. The effect of pH, temperature, liquid/solid ratio (g/cm3) and lead concentration on the sorption capacity of yeasts chemically modified with cubic silsesquioxane (YS) was analyzed. Additionally, the toxicity of lead on the neuronal activity was also investigated in order to assess whether the damage caused by the Pb2+ ion is reversible or not. The YS is highly promissory as sorbent of lead in high concentrations (100 and 500 ppm), reaching high efficiency in short contact times (15 min), and at the natural pH (4) of the Pb2+ solution and room temperature. The best sorption obtained was 82% removal and 248 mg/g with 500 cm3/g sorbent, pH 4, room temperature and contact time of 15 min. Besides, such high efficiencies are obtained with low quantities of biosorbent, when compared with other similar materials. The impact of lead on neuronal function was studied by measuring autofluorescence signals, associated with changes in cellular metabolism, at the hippocampal CA3 area in brain slices. In this toxicity tests, the effect of low concentrations of lead (1 and 3 μM) on neuronal activity was evaluated. After removal of the lead, the irreversibility of the observed changes can be verified, which suggests the existence of neuronal damages.en
dc.description.affiliationFaculdade de Engenharia de Ilha Solteira Univ. Estadual Paulista-UNESP Departamento de Física e Química, 56 CEP, Av. Brasil Centro
dc.description.affiliationCIEPQPF- Research Center of Chemical Process Engineering and Forest Products Department of Chemical Engineering University of Coimbra
dc.description.affiliationCNC- Center for Neuroscience and Cell Biology and Department of Physics University of Coimbra
dc.description.affiliationUnespFaculdade de Engenharia de Ilha Solteira Univ. Estadual Paulista-UNESP Departamento de Física e Química, 56 CEP, Av. Brasil Centro
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.format.extent43-50
dc.identifierhttp://dx.doi.org/10.1016/j.jbiotec.2017.10.015
dc.identifier.citationJournal of Biotechnology, v. 264, p. 43-50.
dc.identifier.doi10.1016/j.jbiotec.2017.10.015
dc.identifier.file2-s2.0-85032379602.pdf
dc.identifier.issn1873-4863
dc.identifier.issn0168-1656
dc.identifier.scopus2-s2.0-85032379602
dc.identifier.urihttp://hdl.handle.net/11449/175395
dc.language.isoeng
dc.relation.ispartofJournal of Biotechnology
dc.relation.ispartofsjr0,929
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAutofluorescence
dc.subjectBiosorption
dc.subjectLead
dc.subjectNeuronal toxicity
dc.subjectSilsesquioxane
dc.subjectToxicity
dc.subjectYeast
dc.titleA study of bio-hybrid silsesquioxane/yeast: Biosorption and neuronal toxicity of leaden
dc.typeArtigo

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