Down-regulation of MyoD gene expression in rat diaphragm muscle with heart failure

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dc.contributor.authorLopes, Francis da Silva
dc.contributor.authorCarvalho, Robson Francisco [UNESP]
dc.contributor.authorCampos, Gerson Eduardo Rocha
dc.contributor.authorSugizaki, Mario Matheus [UNESP]
dc.contributor.authorPadovani, Carlos Roberto [UNESP]
dc.contributor.authorNogueira, Celia Regina [UNESP]
dc.contributor.authorCicogna, Antonio Carlos [UNESP]
dc.contributor.authorDal Pai-Silva, Maeli [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.contributor.institutionUniversidade do Oeste Paulista (UNOESTE)
dc.date.accessioned2014-05-20T13:52:16Z
dc.date.available2014-05-20T13:52:16Z
dc.date.issued2008-06-01
dc.description.abstractDiaphragm myopathy has been described in patients with heart failure (HF), with alterations in myosin heavy chains (MHC) expression. The pathways that regulate MHC expression during HF have not been described, and myogenic regulatory factors (MRFs) may be involved. The purpose of this investigation was to determine MRF mRNA expression levels in the diaphragm. Diaphragm muscle from both HF and control Wistar rats was studied when overt HF had developed, 22 days after monocrotaline administration. MyoD, myogenin and MRF4 gene expression were determined by RT-PCR and MHC isoforms by polyacrylamide gel electrophoresis. Heart failure animals presented decreased MHC IIa/IIx protein isoform and MyoD gene expression, without altering MHC I, IIb, myogenin and MRF4. Our results show that in HF, MyoD is selectively down-regulated, which might be associated with alterations in MHC IIa/IIx content. These changes are likely to contribute to the diaphragm myopathy caused by HF.en
dc.description.affiliationUNESP, Dept Morphol, BR-18618000 Botucatu, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Biol Celular, Campinas, SP, Brazil
dc.description.affiliationUniv Estadual Campinas, Dept Anat, Campinas, SP, Brazil
dc.description.affiliationUniv Oeste Paulista, Dept Fisioterapia, Presidente Prudente, Brazil
dc.description.affiliationUnespUNESP, Dept Morphol, BR-18618000 Botucatu, SP, Brazil
dc.format.extent216-222
dc.identifierhttp://dx.doi.org/10.1111/j.1365-2613.2008.00587.x
dc.identifier.citationInternational Journal of Experimental Pathology. Malden: Wiley-blackwell, v. 89, n. 3, p. 216-222, 2008.
dc.identifier.doi10.1111/j.1365-2613.2008.00587.x
dc.identifier.issn0959-9673
dc.identifier.lattes5406518799128485
dc.identifier.lattes8727897080522289
dc.identifier.orcid0000-0002-4901-7714
dc.identifier.urihttp://hdl.handle.net/11449/18680
dc.identifier.wosWOS:000255603000041
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofInternational Journal of Experimental Pathology
dc.relation.ispartofjcr1.938
dc.relation.ispartofsjr0,712
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectdiaphragm muscleen
dc.subjectheart failureen
dc.subjectmyogenic regulatory factorsen
dc.subjectmyosin heavy chainen
dc.subjectWistar ratsen
dc.titleDown-regulation of MyoD gene expression in rat diaphragm muscle with heart failureen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-blackwell
unesp.author.lattes5406518799128485
unesp.author.lattes8727897080522289[5]
unesp.author.lattes9418970103564137[7]
unesp.author.lattes7607038776901890[6]
unesp.author.orcid0000-0002-4901-7714[2]
unesp.author.orcid0000-0002-4402-6523[7]
unesp.author.orcid0000-0002-7719-9682[5]
unesp.author.orcid0000-0002-4014-0660[6]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Botucatupt

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