Fibrin biopolymer as scaffold candidate to treat bone defects in rats

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dc.contributor.authorCassaro, Claudia Vilalva [UNESP]
dc.contributor.authorJustulin, Luis Antonio [UNESP]
dc.contributor.authorDe Lima, Patrícia Rodrigues [UNESP]
dc.contributor.authorDe Assis Golim, Marjorie [UNESP]
dc.contributor.authorBiscola, Natália Perussi [UNESP]
dc.contributor.authorDe Castro, Mateus Vidigal
dc.contributor.authorDe Oliveira, Alexandre Leite Rodrigues
dc.contributor.authorDoiche, Danuta Pulz [UNESP]
dc.contributor.authorPereira, Elenize Jamas [UNESP]
dc.contributor.authorFerreira, Rui Seabra [UNESP]
dc.contributor.authorBarraviera, Benedito [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2020-12-12T01:47:11Z
dc.date.available2020-12-12T01:47:11Z
dc.date.issued2019-01-01
dc.description.abstractBackground: Bone tissue repair remains a challenge in tissue engineering. Currently, new materials are being applied and often integrated with live cells and biological scaffolds. The fibrin biopolymer (FBP) proposed in this study has hemostatic, sealant, adhesive, scaffolding and drug-delivery properties. The regenerative potential of an association of FBP, biphasic calcium phosphate (BCP) and mesenchymal stem cells (MSCs) was evaluated in defects of rat femurs. Methods: Adult male Wistar rats were submitted to a 5-mm defect in the femur. This was filled with the following materials and/or associations: BPC; FBP and BCP; FBP and MSCs; and BCP, FBP and MSCs. Bone defect without filling was defined as the control group. Thirty and sixty days after the procedure, animals were euthanatized and subjected to computed tomography, scanning electron microscopy and qualitative and quantitative histological analysis. Results: It was shown that FBP is a suitable scaffold for bone defects due to the formation of a stable clot that facilitates the handling and optimizes the surgical procedures, allowing also cell adhesion and proliferation. The association between the materials was biocompatible. Progressive deposition of bone matrix was higher in the group treated with FBP and MSCs. Differentiation of mesenchymal stem cells into osteogenic lineage was not necessary to stimulate bone formation. Conclusions: FBP proved to be an excellent scaffold candidate for bone repair therapies due to application ease and biocompatibility with synthetic calcium-based materials. The satisfactory results obtained by the association of FBP with MSCs may provide a more effective and less costly new approach for bone tissue engineering.en
dc.description.affiliationCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.affiliationBotucatu Medical School (FMB) São Paulo State University (UNESP)
dc.description.affiliationExtracellular Matrix Laboratory Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP)
dc.description.affiliationFlow Cytometry Laboratory Blood Center Botucatu Medical School (FMB) São Paulo State University (UNESP)
dc.description.affiliationDepartment of Structural and Functional Biology Biosciences Institute (IB) University of Campinas (UNICAMP)
dc.description.affiliationDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Husbandry São Paulo State University (UNESP)
dc.description.affiliationUnespCenter for the Study of Venoms and Venomous Animals (CEVAP) São Paulo State University (UNESP)
dc.description.affiliationUnespBotucatu Medical School (FMB) São Paulo State University (UNESP)
dc.description.affiliationUnespExtracellular Matrix Laboratory Botucatu Biosciences Institute (IBB) São Paulo State University (UNESP)
dc.description.affiliationUnespFlow Cytometry Laboratory Blood Center Botucatu Medical School (FMB) São Paulo State University (UNESP)
dc.description.affiliationUnespDepartment of Animal Reproduction and Veterinary Radiology School of Veterinary Medicine and Animal Husbandry São Paulo State University (UNESP)
dc.identifierhttp://dx.doi.org/10.1590/1678-9199-jvatitd-2019-0027
dc.identifier.citationJournal of Venomous Animals and Toxins Including Tropical Diseases, v. 25.
dc.identifier.doi10.1590/1678-9199-jvatitd-2019-0027
dc.identifier.fileS1678-91992019000100320.pdf
dc.identifier.issn1678-9199
dc.identifier.issn1678-9180
dc.identifier.scieloS1678-91992019000100320
dc.identifier.scopus2-s2.0-85075516328
dc.identifier.urihttp://hdl.handle.net/11449/199709
dc.language.isoeng
dc.relation.ispartofJournal of Venomous Animals and Toxins Including Tropical Diseases
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiomaterials
dc.subjectBiphasic calcium
dc.subjectBone regeneration
dc.subjectFibrin biopolymer
dc.subjectFibrin sealant
dc.subjectMesenchymal stem cells
dc.subjectPhosphate
dc.titleFibrin biopolymer as scaffold candidate to treat bone defects in ratsen
dc.typeArtigo
unesp.author.lattes6840524602748457[11]
unesp.author.orcid0000-0002-9855-5594[11]

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