Ultra-structure and histochemistry of digestive cells of Podisus nigrispinus (Hemiptera: Pentatomidae) fed with prey reared on bt-cotton

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dc.contributor.authorda Cunha, Franklin M.
dc.contributor.authorCaetano, Flávio Henrique [UNESP]
dc.contributor.authorWanderley-Teixeira, Valeria
dc.contributor.authorTorres, Jorge B.
dc.contributor.authorTeixeira, Alvaro A. C.
dc.contributor.authorAlves, Luiz C.
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:55:12Z
dc.date.available2014-05-20T13:55:12Z
dc.date.issued2012-02-01
dc.description.abstractThe toxic effects of Bt proteins from the body of the prey/host to predators and parasitoids have been investigated as a route of impact on non-target insects of Bt crops. This research aimed to analyze ultrastructural changes and histochemistry in digestive cells of the middle region of the midgut of Podisus nigrispinus fed, since its second instar, with Spodoptera frugiperda reared on Bt cotton variety Acala90 B, which expresses the toxin Cry 1Ac, and its non-Bt isoline Acala 90. Fragments of the midgut of P. nigrispinus were analyzed by electron microscopy. For the histochemical analysis, Bromophenol Blue, Periodic Acid Schiff von Kossa, Alcian Blue pH 2.5 and Sudan Black were used. The Cry1Ac toxin of Bt cotton ingested by S. frugiperda promotes a disorganization in the perimicrovillar matrix of P. nigrispinus (third trophic level), thus generating ultrastructural changes in the digestive cells, as elongation of microvilli, presence of spherocrystals and granules of different electron densities, in addition to altering the distribution pattern of glycogen, lipids and calcium of these cells in the median region of the midgut. Thus, we conclude that species moderately susceptible to Cry1Ac toxin, such as S. frugiperda, can acquire this toxin and expose it to P. nigrispinus, which can interfere with your ability to predation. (C) 2011 Elsevier Ltd. All rights reserved.en
dc.description.affiliationRural Fed Univ Pernambuco, Dept Agron Entomol, BR-52171900 Recife, PE, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.affiliationRural Fed Univ Pernambuco, Dept Morphol & Physiol, BR-52171900 Recife, PE, Brazil
dc.description.affiliationUniversidade Federal de Pernambuco (UFPE), Aggeu Magalhaes Res Ctr, LIKA, BR-50670420 Recife, PE, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Biol, BR-13506900 Rio Claro, SP, Brazil
dc.description.sponsorshipCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.format.extent245-250
dc.identifierhttp://dx.doi.org/10.1016/j.micron.2011.08.006
dc.identifier.citationMicron. Oxford: Pergamon-Elsevier B.V. Ltd, v. 43, n. 2-3, p. 245-250, 2012.
dc.identifier.doi10.1016/j.micron.2011.08.006
dc.identifier.issn0968-4328
dc.identifier.lattes4396826019535898
dc.identifier.urihttp://hdl.handle.net/11449/19747
dc.identifier.wosWOS:000299605300020
dc.language.isoeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relation.ispartofMicron
dc.relation.ispartofjcr1.728
dc.relation.ispartofsjr0,624
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectBacillus thuringiensisen
dc.subjectPredatoren
dc.subjectMorphologyen
dc.subjectTransgenic planten
dc.subjectHistologyen
dc.titleUltra-structure and histochemistry of digestive cells of Podisus nigrispinus (Hemiptera: Pentatomidae) fed with prey reared on bt-cottonen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderPergamon-Elsevier B.V. Ltd
unesp.author.lattes4396826019535898
unesp.author.orcid0000-0002-0717-6493[4]
unesp.campusUniversidade Estadual Paulista (Unesp), Instituto de Biociências, Rio Claropt

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