Expression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasiveness

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dc.contributor.authorSilva, Tarsia G. [UNESP]
dc.contributor.authorCrispim, Janaina C.O.
dc.contributor.authorMiranda, Fabiana A.
dc.contributor.authorHassumi, Marcela K.
dc.contributor.authorde Mello, Júlia M.Y. [UNESP]
dc.contributor.authorSimões, Renata T.
dc.contributor.authorSouto, Francisco
dc.contributor.authorSoares, Edson G.
dc.contributor.authorDonadi, Eduardo A.
dc.contributor.authorSoares, Christiane Pienna [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade Federal do Rio Grande do Norte (UFRN)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionFederal University of Mato Grosso
dc.date.accessioned2014-05-27T11:26:17Z
dc.date.available2014-05-27T11:26:17Z
dc.date.issued2011-12-01
dc.description.abstractIntroduction: HLA-G and HLA-E are two nonclassical class I molecules, which have been well recognized as modulators of innate and adaptive immune responses, and the expression of these molecules in virus infected cells has been associated with subversion of the immune response. Objective: In this study we performed a cross-sectional study, systematically comparing the expression of HLA-G and HLA-E in benign, premalignant and malignant laryngeal lesions, correlating with demographic and clinical variables and with the presence of high-risk and low-risk HPV types. Materials and methods: Laryngeal lesions were collected from 109 patients and stratified into 27 laryngeal papillomas, 17 dysplasias, 10 in situ laryngeal carcinomas, 27 laryngeal carcinomas without metastases, 28 laryngeal carcinomas with metastasis along with their respective draining cervical lymph nodes, and 10 normal larynx specimens. The expression of HLA-G and HLA-E molecules was determined by immunohistochemistry. HPV DNA detection and typing was performed using generic and specific primers. Results: HLA nonclassical molecules showed a distinct distribution pattern, according to the larynx lesion grade. HLA-G expression increased in benign and premalignant lesions, and gradually decreased in invasive carcinomas and in respective draining cervical lymph nodes. Conversely, HLA-E expression increased as far as lesion grade increased, including increased molecule expression in the draining lymph nodes of malignant lesions. Only 17 (15.6%) patients were HPV DNA positive. Conclusions: Overexpression of HLA-E and underexpression of HLAG appear to be good markers for malignant larynx lesion.en
dc.description.affiliationDepartment of Clinical Analysis School of Pharmaceutical Sciences UNESP, SP
dc.description.affiliationDepartment of Clinical and Toxicology Analysis Faculty of Pharmaceutical Sciences, UFRN, RN
dc.description.affiliationDivision of Clinical Immunology Department of Medicine Faculty of Medicine of Ribeirão Preto, University of São Paulo, SP
dc.description.affiliationDepartment of Pathology Faculty of Medicine of Ribeirão Preto University of São Paulo-USP, SP
dc.description.affiliationDepartment of Internal Medicine Federal University of Mato Grosso, Cuiabá, Mato Grosso
dc.description.affiliationUnespDepartment of Clinical Analysis School of Pharmaceutical Sciences UNESP, SP
dc.format.extent1487-1497
dc.identifierhttp://www.hh.um.es/Abstracts/Vol_26/26_12/26_12_1487.htm
dc.identifier.citationHistology and Histopathology, v. 26, n. 12, p. 1487-1497, 2011.
dc.identifier.file2-s2.0-80053940545.pdf
dc.identifier.issn0213-3911
dc.identifier.lattes1768025290373669
dc.identifier.orcid0000-0003-1740-7360
dc.identifier.scopus2-s2.0-80053940545
dc.identifier.urihttp://hdl.handle.net/11449/72978
dc.language.isoeng
dc.relation.ispartofHistology and Histopathology
dc.relation.ispartofjcr2.015
dc.relation.ispartofsjr0,672
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectBiomarkers
dc.subjectHLA-E
dc.subjectHLA-G
dc.subjectHPV
dc.subjectLaryngeal lesions
dc.subjectHLA antigen class 1
dc.subjectHLA E antigen
dc.subjectHLA G antigen
dc.subjectHLA-E antigen
dc.subjecttumor marker
dc.subjectvirus DNA
dc.subjectadult
dc.subjectaged
dc.subjectanalysis of variance
dc.subjectbiopsy
dc.subjectBrazil
dc.subjectcancer invasion
dc.subjectcarcinoma
dc.subjectcarcinoma in situ
dc.subjectcomparative study
dc.subjectcross-sectional study
dc.subjectfemale
dc.subjectgenetics
dc.subjecthuman
dc.subjectimmunohistochemistry
dc.subjectimmunology
dc.subjectlarynx tumor
dc.subjectlymph node
dc.subjectlymph node metastasis
dc.subjectmale
dc.subjectmetastasis
dc.subjectmiddle aged
dc.subjectpapilloma
dc.subjectPapilloma virus
dc.subjectpathology
dc.subjectpolymerase chain reaction
dc.subjectprecancer
dc.subjectrisk
dc.subjectvirology
dc.subjectAdult
dc.subjectAged
dc.subjectAnalysis of Variance
dc.subjectBiopsy
dc.subjectCarcinoma
dc.subjectCarcinoma in Situ
dc.subjectCross-Sectional Studies
dc.subjectDNA, Viral
dc.subjectFemale
dc.subjectHistocompatibility Antigens Class I
dc.subjectHLA-G Antigens
dc.subjectHumans
dc.subjectImmunohistochemistry
dc.subjectLaryngeal Neoplasms
dc.subjectLymph Nodes
dc.subjectLymphatic Metastasis
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectNeoplasm Invasiveness
dc.subjectOdds Ratio
dc.subjectPapilloma
dc.subjectPapillomaviridae
dc.subjectPolymerase Chain Reaction
dc.subjectPrecancerous Conditions
dc.subjectTumor Markers, Biological
dc.subjectHuman papillomavirus
dc.titleExpression of the nonclassical HLA-G and HLA-E molecules in laryngeal lesions as biomarkers of tumor invasivenessen
dc.typeArtigo
dcterms.licensehttp://revistas.um.es/analesps/about/submissions#copyrightNotice
unesp.author.lattes1768025290373669[10]
unesp.author.orcid0000-0003-1740-7360[10]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Ciências Farmacêuticas, Araraquarapt

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