Compartmentalization of pro-inflammatory cytokine levels in renal-transplanted pregnant women

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dc.contributor.authorCorrea-Silva, Simone [UNESP]
dc.contributor.authorPrado, Karen Matias Do
dc.contributor.authorOliveira, Leandro Gustavo de [UNESP]
dc.contributor.authorOno, Erica
dc.contributor.authorCamara, Niels Olsen Saraiva [UNESP]
dc.contributor.authorBevilacqua, Estela
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:30:49Z
dc.date.available2014-05-27T11:30:49Z
dc.date.issued2013-10-01
dc.description.abstractObjective: We evaluated whether chronic exposure to immunosuppression in transplant recipients modulate the placental inflammatory cytokine levels associated to gestational tolerance mechanisms. Methods: Serum samples were collected from 12 renal transplanted pregnant under immunosuppressive regimen treatment and 10 healthy women in second/third trimester of gestation. Term placental tissues (decidua and chorionic villi) were also obtained after elective caesarean. Serum IL-1β, IL-6, IL-8, IL-12p70 and TNF-α were measured, as also in placental homogenates, by Cytometric Bead Array (CBA) combined with flow cytometry and, TGF-β and IL-18 were measured by ELISA. Results: Serum levels of IL-6 (p=0.0001) and TNF-α (0.0112) were higher in the 2nd and 3rd trimesters and in decidua the spectrum of increased pro inflammatory cytokines was wider: IL-1β (p=0.0001), IL-6 (p=0.0001), IL-8 (p=0.0001), IL-12p70 (p=0.0001), TGF-β (p=0.0089) and TNF-α (p=0.0002). TGF-β1 was particularly increased in decidual compartment (p=0.001). In the chorionic villous, pro inflammatory profile also were maintained. High IL-1β (p=0.0001), IL-6 (p=0.0001), IL-8 (p=0.0001) and TNF-α (p=0.0001) levels establish a similar pattern to that seem in decidua. Conclusion: Immunosuppressors may impair the immune response, but when associated with pregnancy the cytokine levels seems to shift a proinflammatory profile in placental compartments, which might also impact on the gestational outcomes in transplanted mothers. © 2013 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted.en
dc.description.affiliationDepartment of Cell and Developmental Biology Institute of Biomedical Sciences University of São Paulo, Av Prof Lineu Prestes, 1524-Butantan-05508-900 SP
dc.description.affiliationGraduate Program in Gynecology, Obstetrics and Mastology Botucatu Medical School UNESP-São Paulo State University, SP
dc.description.affiliationDepartment of Immunology Institute of Biomedical Sciences University of São Paulo, SP
dc.description.affiliationDepartment of Obstetrics Federal University of São Paulo, SP
dc.description.affiliationUnespGraduate Program in Gynecology, Obstetrics and Mastology Botucatu Medical School UNESP-São Paulo State University, SP
dc.format.extent1468-1473
dc.identifierhttp://dx.doi.org/10.3109/14767058.2013.784736
dc.identifier.citationJournal of Maternal-Fetal and Neonatal Medicine, v. 26, n. 15, p. 1468-1473, 2013.
dc.identifier.doi10.3109/14767058.2013.784736
dc.identifier.issn1476-7058
dc.identifier.issn1476-4954
dc.identifier.lattes0720794820325913
dc.identifier.scopus2-s2.0-84884536086
dc.identifier.urihttp://hdl.handle.net/11449/76769
dc.identifier.wosWOS:000324588200002
dc.language.isoeng
dc.relation.ispartofJournal of Maternal-Fetal and Neonatal Medicine
dc.relation.ispartofjcr1.493
dc.relation.ispartofsjr0,714
dc.relation.ispartofsjr0,714
dc.rights.accessRightsAcesso restrito
dc.sourceScopus
dc.subjectCytokines
dc.subjectImmune response
dc.subjectImmunosuppressive drugs
dc.subjectInflammation
dc.subjectPlacenta
dc.titleCompartmentalization of pro-inflammatory cytokine levels in renal-transplanted pregnant womenen
dc.typeArtigo
dcterms.licensehttp://informahealthcare.com/userimages/ContentEditor/1255620309227/Copyright_And_Permissions.pdf
unesp.author.lattes0720794820325913
unesp.author.orcid0000-0003-4178-4625[6]
unesp.author.orcid0000-0001-5436-1248[5]
unesp.campusUniversidade Estadual Paulista (Unesp), Faculdade de Medicina, Botucatupt

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