Now showing items 1-3 of 3
Purification and Characterization of the alpha-Glucosidase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI 756
(Journal of Microbiology, 2010-08-01) [Artigo]
An alpha-glucosidase enzyme produced by the fungus Thermoascus aurantiacus CBMAI 756 was purified by ultra filtration, ammonium sulphate precipitation, and chromatography using Q Sepharose, Sephacryl S-200, and Superose ...
Influence of Different Substrates on the Production of a Mutant Thermostable Glucoamylase in Submerged Fermentation
(Applied Biochemistry and Biotechnology, 2011-01-01) [Artigo]
Three mutations, Ser54 -> Pro, Thr314 -> Ala, and His415 -> Tyr, were identified in Aspergillus awamori glucoamylase gene expressed by Saccharomyces cerevisiae. The mutant glucoamylase (GA) was substantially more thermostable ...
Partial purification, immobilization and preliminary biochemical characterization of lipases from Rhizomucor pusillus
(Advances in Enzyme Research, 2013) [Artigo]
Lipases have important applications in biotechnological processes, motivating us to produce, purify, immobilize and perform a biochemical characterization of the lipase from Rhizomucor pusillus. The fungus was cultivated ...