Now showing items 11-20 of 21
FUNCTIONAL ALPHA-TROPOMYOSIN PRODUCED IN ESCHERICHIA-COLI - A DIPEPTIDE EXTENSION CAN SUBSTITUTE THE AMINO-TERMINAL ACETYL GROUP
(Journal of Biological Chemistry, 1994-04-08) [Artigo]
Unlike the muscle protein, alpha-tropomyosin expressed in Escherichia coli does not bind actin, does not exhibit head-to-tail polymerization, and does not inhibit actomyosin ATPase activity in the absence of troponin. The ...
Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers
(British Poultry Science, 1996-05-01) [Artigo]
1. The synthesis of heat shock protein 70 (Hsp70) mRNA and the expression of Hsp70 in the liver of broiler chickens submitted to acute heat stress (35 degrees C for 5 h) was investigated.2. Hsp70 expression was detected ...
Short communication: Resazurin reducing time as an indicator of Bradyrhizobium viable cell count
(World Journal of Microbiology and Biotechnology, 1998-01-01) [Artigo]
A method based on the use of resazurin (RSZ) is described to determine the number of viable Bradyrhizobium japonicum cells in culture medium. The observation of RSZ reduction can be done spectrophotometrically or visually. ...
Electrophoretic patterns of lipopolysaccharides and antigenic analysis of soybean bradyrhizobia
(World Journal of Microbiology and Biotechnology, 1999-01-01) [Artigo]
Several serological methods have been used for the characterization and identification of soybean bradyrhizobia. However, some problem were non-reactivity of certain strains and cross-reactivity among others. Since ...
Recombinant chicken tropomyosin produced in yeast cells
(FASEB Journal, 1999-04-23) [Resumo]
Allosteric modulation of pyrophosphatase activity of rat osseous plate alkaline phosphatase by magnesium ions
(International Journal of Biochemistry & Cell Biology, 1998-01-01) [Artigo]
Pyrophosphatase activity of rat osseous plate alkaline phosphatase was studied at different concentrations of calcium and magnesium ions. with the aim of characterizing the modulation of enzyme activity by these metals. ...
Use of lipopolysaccharides to characterize Bradyrhizobium spp.
(Soil Biology and Biochemistry, 1996-09-01) [Artigo]
Three methods of extraction of lipopolysaccharide (LPS) were compared-the conventional hot phenol-water method with 40% phenol, a modified form of this method using 10% phenol, and the hot saline method. Good recovery of ...
ALLOSTERIC MODULATION BY ATP, CALCIUM AND MAGNESIUM-IONS OF RAT OSSEOUS PLATE ALKALINE-PHOSPHATASE
(Biochimica Et Biophysica Acta, 1993-09-03) [Artigo]
Alkaline phosphatase from rat osseous plate is allosterically modulated by ATP, calcium and magnesium at pH 7.5. At pH 9.4, the hydrolysis of ATP and PNPP follows Michaelis-Menten kinetics with K0.5 values of 154 muM and ...
OSSEOUS PLATE ALKALINE-PHOSPHATASE IS ANCHORED BY GPI
(Brazilian Journal of Medical and Biological Research, 1994-02-01) [Artigo]
Alkaline phosphatase activity was released up to 100% from the membrane by using 0.1 U of phosphatidylinositol-specific phospholipase C from B. thuringiensis. The Mr of solubilized enzyme was 145,000 by Sephacryl S-300 gel ...
Hepatic concentration of heat shock protein 70 kD (Hsp70) in broilers subjected to different thermal treatments
(British Poultry Science, 1999-05-01) [Artigo]
1. The relationship between repeated thermal treatments and hepatic synthesis of Hsp 70 was studied in broiler chickens.2. Sixty broilers were submitted to 5 different treatments (12 birds each) from day 1 to day 42. Four ...