HPLC determination of flumethrin, deltamethrin, cypermethrin, and cyhalothrin residues in the milk and blood of lactating dairy cows

A procedure to determine residue concentrations of synthetic pyrethroid insecticides (flumethrin, deltamethrin, cypermethrin and cyhalothrin) in the milk and blood of lactating dairy cows was developed. Extraction was performed with acetonitrile, n-hexane partitioning, and silica gel column cleanup with n-hexane and diethyl ether. Analysis was carried out by high- performance liquid chromatography and ultraviolet detection. Recovery of the four pyrethroids averaged 78 to 91% with a minimum detectable concentration of 0.001 mg/kg. The method was reproducible and sensitive.

Keywords

cipermethrin, cyhalothrin, deltamethrin, flumethrin, insecticide, pyrethroid, chemical analysis, cow, food contamination, high performance liquid chromatography, lactation, milk, nonhuman, ultraviolet radiation, Animals, Cattle, Chromatography, High Pressure Liquid, Female, Insecticides, Lactation, Milk, Nitriles, Pesticide Residues, Pyrethrins, Reproducibility of Results, Sensitivity and Specificity, Spectrophotometry, Ultraviolet