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Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)

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dc.contributor.authorGomes, Eleni [UNESP]
dc.contributor.authorSerzedello, Alcides [UNESP]
dc.contributor.authorDa Silva, Roberto [UNESP]
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.date.accessioned2022-04-28T19:54:36Z
dc.date.available2022-04-28T19:54:36Z
dc.date.issued1998-12-01
dc.description.abstractA RNase of Aspergillus flavlpes (IZ:1501) was purified from culture medium by chromatography on DEAE-cellulose and Sephadex G50 columns, after 96 h of cultivation. The molecular weight of the RNase was estimated to be 15 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 55°C, respectively. Catalytic activity was inhibited by Hg2+, Ag+, Fe3+, Co2+ and Zn2+. The enzyme showed guanosine specificity producing only 3′-GMP from yeast RNA.en
dc.description.affiliationLab. Bioquim. dos Processus M. Departamento de Biologia Univ. Estadual Paulista-IBILCE, CP 163, CEP 15054-000
dc.description.affiliationDepto. de Quim. e Geociências UNESP-IBILCE
dc.description.affiliationDepto. de Bioquim. e Microbiologia UNESP, Rio Claro, SP
dc.description.affiliationUnespLab. Bioquim. dos Processus M. Departamento de Biologia Univ. Estadual Paulista-IBILCE, CP 163, CEP 15054-000
dc.description.affiliationUnespDepto. de Quim. e Geociências UNESP-IBILCE
dc.description.affiliationUnespDepto. de Bioquim. e Microbiologia UNESP, Rio Claro, SP
dc.format.extent39-50
dc.identifier.citationMicrobios, v. 96, n. 383, p. 39-50, 1998.
dc.identifier.issn0026-2633
dc.identifier.scopus2-s2.0-0032262633
dc.identifier.urihttp://hdl.handle.net/11449/224099
dc.language.isoeng
dc.relation.ispartofMicrobios
dc.sourceScopus
dc.subjectAspergillus
dc.subjectNucleotides
dc.subjectRibonuclease
dc.titlePurification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ:1501)en
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBioquímica e Microbiologia - IBpt
unesp.departmentQuímica e Ciências Ambientais - IBILCEpt

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São José do Rio Preto - IBILCE - Instituto de Biociências, Letras e Ciências Exatas
Rio Claro - IB - Instituto de Biociências