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Isolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtzii

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dc.contributor.authorDevitt, Thomas J.
dc.contributor.authorPereira, Ricardo
dc.contributor.authorJakkula, Lakshmi
dc.contributor.authorAlexandrino, Joao [UNESP]
dc.contributor.authorBardeleben, Carolyne
dc.contributor.authorMoritz, Craig
dc.contributor.institutionUniv Calif Berkeley
dc.contributor.institutionUniv Porto
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Calif Los Angeles
dc.date.accessioned2014-05-20T15:31:17Z
dc.date.available2014-05-20T15:31:17Z
dc.date.issued2009-05-01
dc.description.abstractWe developed 15 new polymorphic microsatellites for the plethodontid salamander Ensatina eschscholtzii. Loci were isolated from a genomic library from Ensatina eschscholtzii xanthoptica enriched for (AAAG)(n) repetitive elements. The number of alleles per locus ranged from 4 to 20 (mean 9) in the sampled population. Observed heterozygosity ranged from 0.37 to 1. None of the loci deviated from Hardy-Weinberg equilibrium or showed significant linkage disequilibrium after a Bonferroni correction for multiple comparisons. All loci amplified in the six other subspecies of the Ensatina eschscholtzii complex. These new markers will prove useful in measuring gene flow and population structure as well as patterns of mating and sperm use in Ensatina.en
dc.description.affiliationUniv Calif Berkeley, Museum Vertebrate Zool, Berkeley, CA 94720 USA
dc.description.affiliationUniv Calif Berkeley, Dept Integrat Biol, Berkeley, CA 94720 USA
dc.description.affiliationUniv Porto, CIBIO, Ctr Invest Biodiversidade & Recursos Genet, P-4485661 Vairao, Portugal
dc.description.affiliationUniv Calif Berkeley, Lawrence Berkeley Lab, Berkeley, CA 94720 USA
dc.description.affiliationUniv Estadual Paulista, Inst Biociencias, Dept Zool, BR-13506900 São Paulo, Brazil
dc.description.affiliationUniv Calif Los Angeles, Dept Ecol & Evolutionary Biol, Los Angeles, CA 90095 USA
dc.description.affiliationUnespUniv Estadual Paulista, Inst Biociencias, Dept Zool, BR-13506900 São Paulo, Brazil
dc.description.sponsorshipNSF
dc.description.sponsorshipIdNSF: DEB 0641078
dc.format.extent966-969
dc.identifierhttp://dx.doi.org/10.1111/j.1755-0998.2009.02518.x
dc.identifier.citationMolecular Ecology Resources. Malden: Wiley-blackwell Publishing, Inc, v. 9, n. 3, p. 966-969, 2009.
dc.identifier.doi10.1111/j.1755-0998.2009.02518.x
dc.identifier.issn1755-098X
dc.identifier.urihttp://hdl.handle.net/11449/40467
dc.identifier.wosWOS:000265897200083
dc.language.isoeng
dc.publisherWiley-Blackwell Publishing, Inc
dc.relation.ispartofMolecular Ecology Resources
dc.relation.ispartofjcr7.059
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectEnsatinaen
dc.subjectmicrosatelliteen
dc.subjectPlethodontidaeen
dc.subjectplethodontiden
dc.subjectring speciesen
dc.subjectsalamanderen
dc.titleIsolation and characterization of 15 polymorphic microsatellites in the Plethodontid salamander Ensatina eschscholtziien
dc.typeArtigo
dcterms.licensehttp://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1601-0825/homepage/ForAuthors.html
dcterms.rightsHolderWiley-blackwell Publishing, Inc
dspace.entity.typePublication
unesp.author.orcid0000-0003-2427-0964[4]
unesp.author.orcid0000-0002-8076-4822[2]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.departmentZoologia - IBpt

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Rio Claro - IB - Instituto de Biociências