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Crystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seeds

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dc.contributor.authorMoreno, FBMB
dc.contributor.authorDelatorre, P.
dc.contributor.authorFreitas, B. T.
dc.contributor.authorRocha, BAM
dc.contributor.authorSouza, E. P.
dc.contributor.authorFaco, F.
dc.contributor.authorCanduri, F.
dc.contributor.authorCardoso, ALH
dc.contributor.authorFreire, V. N.
dc.contributor.authorLima, J. L.
dc.contributor.authorSampaio, A. H.
dc.contributor.authorCalvete, J. J.
dc.contributor.authorDe Azevedo, W. F.
dc.contributor.authorCavada, B. S.
dc.contributor.institutionUniversidade Federal do Ceará (UFC)
dc.contributor.institutionCSIC
dc.contributor.institutionUniversidade Federal de Pernambuco (UFPE)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniv Reg Cariri
dc.date.accessioned2014-05-20T15:29:51Z
dc.date.available2014-05-20T15:29:51Z
dc.date.issued2004-08-01
dc.description.abstractThe seed lectin from Canavalia gladiata was purified and crystallized. Orthorhombic crystals belonging to space group C222(1) grew within three weeks at 293 K using the hanging-drop vapour-diffusion method. Using synchrotron X-ray radiation, a complete structural data set was collected at 2.3 Angstrom resolution. The preliminary crystal structure of the lectin, determined by molecular replacement, had a correlation coefficient of 0.569 and an R factor of 0.412.en
dc.description.affiliationUniv Fed Ceara, Dept Bioquim & Biol Mol, BR-60451970 Fortaleza, Ceara, Brazil
dc.description.affiliationCSIC, Inst Biomed Valencia, Valencia 46010, Spain
dc.description.affiliationUniv Fed Ceara, Dept Engn Pesca, BR-60451970 Fortaleza, Ceara, Brazil
dc.description.affiliationUniv Fed Pernambuco, Lab Imunopatol Keizo Asami, BR-50670901 Recife, PE, Brazil
dc.description.affiliationUniv Fed Ceara, Dept Fis, BR-60451970 Fortaleza, Ceara, Brazil
dc.description.affiliationFAPESP, CEPID, Ctr Appl Toxinol, BR-05503900 São Paulo, Brazil
dc.description.affiliationUNESP, Dept Fis, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv Reg Cariri, Dept Ciências Biol, Grp Quim Biol, BR-63105000 Crato, Ceara, Brazil
dc.description.affiliationUnespUNESP, Dept Fis, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.format.extent1493-1495
dc.identifierhttp://dx.doi.org/10.1107/S0907444904014489
dc.identifier.citationActa Crystallographica Section D-biological Crystallography. Malden: Wiley-blackwell, v. 60, p. 1493-1495, 2004.
dc.identifier.doi10.1107/S0907444904014489
dc.identifier.issn0907-4449
dc.identifier.urihttp://hdl.handle.net/11449/39332
dc.identifier.wosWOS:000222791700032
dc.language.isoeng
dc.publisherWiley-Blackwell
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallography
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.titleCrystallization and preliminary X-ray diffraction analysis of the lectin from Canavalia gladiata seedsen
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderWiley-Blackwell
dspace.entity.typePublication
unesp.author.orcid0000-0001-5026-3122[12]
unesp.author.orcid0000-0002-5874-6644[8]
unesp.author.orcid|0000-0001-7867-3908[9]
unesp.author.orcid0000-0002-7157-0321[4]
unesp.author.orcid0000-0002-5791-6170[14]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentFísica - IBILCEpt

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São José do Rio Preto - IBILCE - Instituto de Biociências, Letras e Ciências Exatas