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Protein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systems

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dc.contributor.authorJohansson, Hans-Olof
dc.contributor.authorMagaldi, Flavio Musa
dc.contributor.authorFeitosa, Eloi [UNESP]
dc.contributor.authorPessoa, Adalberto
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:02:38Z
dc.date.available2014-05-20T14:02:38Z
dc.date.issued2008-01-12
dc.description.abstractThe partition of hemoglobin, lysozyme and glucose-6-phospate dehydrogenase (G6PDH) in a novel inexpensive aqueous two-phase system (ATPS) composed by poly(ethylene glycol) (PEG) and sodium polyacrylate (NaPA) has been studied. The effect of NaCl and Na2SO4, pH and PEG molecular size on the partitioning has been studied. At high pH (above 9), hemoglobin partitions strongly to the PEG-phase. Although some precipitation of hemoglobin occurs, high recovery values are obtained particularly for lysozyme and G6PDH. The partitioning forces are dominated by the hydrophobic and electrochemical (salt) effects, since the positively charged lysozyme and negatively charged G6PDH partitions to the non-charged PEG and the strongly negatively charged polyacrylate enriched phase, respectively. (c) 2007 Elsevier B.V. All rights reserved.en
dc.description.affiliationUniv São Paulo, Dept Pharmaceut Technol & Biochem, BR-05508000 São Paulo, Brazil
dc.description.affiliationSão Paulo State Univ, Dept Phys, Sao Jose do Rio Preto, Brazil
dc.description.affiliationUnespSão Paulo State Univ, Dept Phys, Sao Jose do Rio Preto, Brazil
dc.format.extent145-153
dc.identifierhttp://dx.doi.org/10.1016/j.chroma.2007.11.071
dc.identifier.citationJournal of Chromatography A. Amsterdam: Elsevier B.V., v. 1178, n. 1-2, p. 145-153, 2008.
dc.identifier.doi10.1016/j.chroma.2007.11.071
dc.identifier.issn0021-9673
dc.identifier.urihttp://hdl.handle.net/11449/22084
dc.identifier.wosWOS:000252477400017
dc.language.isoeng
dc.publisherElsevier B.V.
dc.relation.ispartofJournal of Chromatography A
dc.relation.ispartofjcr3.716
dc.relation.ispartofsjr1,378
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectHemoglobinen
dc.subjectlysozymeen
dc.subjectGlucose-6-phosphate dehydrogenaseen
dc.subjectPolyethylene glycolen
dc.subjectsodium poly(acrylic acid)en
dc.subjectAqueous two-phase systemsen
dc.subjectLiquid-liquid extractionen
dc.titleProtein partitioning in poly(ethylene glycol)/sodium polyacrylate aqueous two-phase systemsen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderElsevier B.V.
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentFísica - IBILCEpt

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São José do Rio Preto - IBILCE - Instituto de Biociências, Letras e Ciências Exatas