Publicação: Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples
dc.contributor.author | Pelizzon, Cristiane [UNESP] | |
dc.contributor.author | da Silva Carvalho, Carolina [UNESP] | |
dc.contributor.author | Caballero, Susana | |
dc.contributor.author | Galetti Junior, Pedro Manoel | |
dc.contributor.author | Sanches, Alexandra | |
dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
dc.contributor.institution | Universidad de los Andes | |
dc.contributor.institution | Universidade Federal de São Carlos (UFSCar) | |
dc.date.accessioned | 2018-12-11T17:10:46Z | |
dc.date.available | 2018-12-11T17:10:46Z | |
dc.date.issued | 2017-03-01 | |
dc.description.abstract | The knowledge of the sex ratio of threatened populations or species is pivotal since sub-optimal sex ratios can affect negatively the population growth and resilience. The vulnerable species, Tapirus terrestris, is rarely studied through traditional field methods and non-invasive genetic analyses have been employed using feces as source of DNA. Here, we evaluated two molecular markers for sex identification of tapirs. Amelogenin gene (AMEL) which is shorter in the Y chromosome (−20pb), failed in the determination of the sex of tapirs, even after the design of specific primers for the species. SRY marker, co-amplified with zinc-finger gene (ZF), had successful produced the sex identity for blood and scat samples. From a total of 45 feaces, 29 had the sex identified after three PCRS (64.4 %). In this way, these molecular markers are reliable tools for sex ratio determination using non-invasive samples of natural populations of this tapirs. | en |
dc.description.affiliation | Departamento de Ecologia Universidade Estadual Julio de Mesquita Filho (UNESP Rio Claro), Av. 24 A | |
dc.description.affiliation | Departamento de Ciencias Biológicas Universidad de los Andes, Carrera 1 No. 18A-10 | |
dc.description.affiliation | Departamento de Genética e Evolução Universidade Federal de São Carlos (UFSCar campus São Carlos), Rodovia Washington Luiz, km 235 | |
dc.description.affiliation | Centro de Ciências da Natureza Universidade Federal de São Carlos (UFSCar campus Lagoa do Sino), Rodovia Lauri Simões de Barros, km 12, SP-189 | |
dc.description.affiliationUnesp | Departamento de Ecologia Universidade Estadual Julio de Mesquita Filho (UNESP Rio Claro), Av. 24 A | |
dc.description.sponsorship | Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) | |
dc.description.sponsorshipId | FAPESP: 2007/04073-1 | |
dc.description.sponsorshipId | FAPESP: 2013/19377-7 | |
dc.format.extent | 17-19 | |
dc.identifier | http://dx.doi.org/10.1007/s12686-016-0607-y | |
dc.identifier.citation | Conservation Genetics Resources, v. 9, n. 1, p. 17-19, 2017. | |
dc.identifier.doi | 10.1007/s12686-016-0607-y | |
dc.identifier.file | 2-s2.0-85015998140.pdf | |
dc.identifier.issn | 1877-7260 | |
dc.identifier.issn | 1877-7252 | |
dc.identifier.scopus | 2-s2.0-85015998140 | |
dc.identifier.uri | http://hdl.handle.net/11449/174365 | |
dc.language.iso | eng | |
dc.relation.ispartof | Conservation Genetics Resources | |
dc.relation.ispartofsjr | 0,430 | |
dc.relation.ispartofsjr | 0,430 | |
dc.rights.accessRights | Acesso aberto | |
dc.source | Scopus | |
dc.subject | Amelogenin | |
dc.subject | Sexing | |
dc.subject | SRY | |
dc.subject | Tapir | |
dc.subject | ZFX/ZFY | |
dc.title | Sex identification of the extant mega mammal, the lowland tapir, Tapirus terrestris (Tapiridae, Mammalia), by means of molecular markers: new outlook for non-invasive samples | en |
dc.type | Artigo | |
dspace.entity.type | Publication | |
unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claro | pt |
unesp.department | Ecologia - IB | pt |
Arquivos
Pacote Original
1 - 1 de 1
Carregando...
- Nome:
- 2-s2.0-85015998140.pdf
- Tamanho:
- 605.56 KB
- Formato:
- Adobe Portable Document Format
- Descrição: