Publicação:
Development and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae)

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dc.contributor.authorManoel, Ricardo de Oliveira [UNESP]
dc.contributor.authorFreitas, Miguel L. M.
dc.contributor.authorBarreto, Mariana A.
dc.contributor.authorMoraes, Mario Luis Teixeira de [UNESP]
dc.contributor.authorSouza, Anete P.
dc.contributor.authorSebbenn, Alexandre M.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionInst Florestal Sao Paulo
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2015-03-18T15:56:21Z
dc.date.available2015-03-18T15:56:21Z
dc.date.issued2014-03-01
dc.description.abstractPremise of the study: Microsatellite primers were developed for the tree species Genipa americana (Rubiaceae) for further population genetic studies.Methods and Results: We identified 144 clones containing 65 repeat motifs from a genomic library enriched for (CT)(8) and (GT)(8) motifs. Primer pairs were developed for 32 microsatellite loci and validated in 40 individuals of two natural G. americana populations. Seventeen loci were polymorphic, revealing from three to seven alleles per locus. The observed and expected heterozygosities ranged from 0.24 to 1.00 and from 0.22 to 0.78, respectively.Conclusions: The 17 primers identified as polymorphic loci are suitable to study the genetic diversity and structure, mating system, and gene flow in G. americanaen
dc.description.affiliationUniv Estadual Paulista, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP, Brazil
dc.description.affiliationInst Florestal Sao Paulo, BR-01059970 Sao Paulo, Brazil
dc.description.affiliationUniv Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP, Brazil
dc.description.sponsorshipFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdFAPESP: 10/19613-4
dc.description.sponsorshipIdCNPq: 473677/2010-5
dc.description.sponsorshipIdFAPESP: 11/01518-8
dc.format.extent3
dc.identifierhttp://dx.doi.org/10.3732/apps.1300084
dc.identifier.citationApplications In Plant Sciences. St Louis: Botanical Soc Amer Inc, v. 2, n. 3, 3 p., 2014.
dc.identifier.doi10.3732/apps.1300084
dc.identifier.fileWOS000342559000005.pdf
dc.identifier.issn2168-0450
dc.identifier.lattes9803426672221802
dc.identifier.urihttp://hdl.handle.net/11449/117525
dc.identifier.wosWOS:000342559000005
dc.language.isoeng
dc.publisherBotanical Soc Amer Inc
dc.relation.ispartofApplications In Plant Sciences
dc.relation.ispartofjcr1.187
dc.relation.ispartofsjr0,746
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.subjectconservation geneticsen
dc.subjectGenipa americanaen
dc.subjectmicrosatellite markersen
dc.subjectpopulation geneticsen
dc.subjectRubiaceaeen
dc.subjecttree speciesen
dc.titleDevelopment and characterization of 32 microsatellite loci in Genipa americana (Rubiaceae)en
dc.typeArtigo
dcterms.rightsHolderBotanical Soc Amer Inc
dspace.entity.typePublication
unesp.author.lattes9803426672221802
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Engenharia, Ilha Solteirapt
unesp.departmentFitotecnia, Tecnologia de Alimentos e Socioeconomia - FEISpt

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Ilha Solteira - FEIS - Faculdade de Engenharia