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Complete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyte

dc.contributor.authorMeirelles, Flávio V.
dc.contributor.authorBordignon, Vilceu
dc.contributor.authorWatanabe, Yeda
dc.contributor.authorWatanabe, Michelle [UNESP]
dc.contributor.authorDayan, André [UNESP]
dc.contributor.authorLôbo, Raysildo B.
dc.contributor.authorGarcia, Joaquim M. [UNESP]
dc.contributor.authorSmith, Lawrence C.
dc.contributor.institutionUniversité de Montréal
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-27T11:20:16Z
dc.date.available2014-05-27T11:20:16Z
dc.date.issued2001-05-22
dc.description.abstractDue to the exclusively maternal inheritance of mitochondria, mitochondrial genotypes can be coupled to a particular nuclear genotype by continuous mating of founder females and their female offspring to males of the desired nuclear genotype. However, backcrossing is a gradual procedure that, apart from being lengthy, cannot ascertain that genetic and epigenetic changes will modify the original nuclear genotype. Animal cloning by nuclear transfer using host ooplasm carrying polymorphic mitochondrial genomes allows, among other biotechnology applications, the coupling of nuclear and mitochondrial genotypes of diverse origin within a single generation. Previous attempts to use Bos taurus oocytes as hosts to transfer nuclei from unrelated species led to the development to the blastocyst stage but none supported gestation to term. Our aim in this study was to determine whether B. taurus oocytes support development of nuclei from the closely related B. indicus cattle and to examine the fate of their mitochondrial genotypes throughout development. We show that indicus:taurus reconstructed oocytes develop to the blastocyst stage and produce live offspring after transfer to surrogate cows. We also demonstrate that, in reconstructed embryos, donor cell-derived mitochondria undergo a stringent genetic drift during early development leading, in most cases, to a reduction or complete elimination of B. indicus mtDNA. These results demonstrate that cross-subspecies animal cloning is a viable approach both for matching diverse nuclear and cytoplasmic genes to create novel breeds of cattle and for rescuing closely related endangered cattle.en
dc.description.affiliationCentre de Recherche en Reproduction Animale Faculté de Médecine Vétérinaire Université de Montréal, Saint-Hyacinthe, Que. J2S 7C6
dc.description.affiliationDepartamento de Genética Faculdade de Medicina-USP, Ribeirão Preto, SP 14049-900
dc.description.affiliationDepartamento de Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias UNESP, Jaboticabal, SP 14884-900
dc.description.affiliationCentre de Recherche en Reproduction Animale Faculté de Médecine Vétérinaire Université de Montréal, 3200, rue Sicotte CP 5000, Saint-Hyacinthe, Que. J2S 7C6
dc.description.affiliationUnespDepartamento de Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias UNESP, Jaboticabal, SP 14884-900
dc.format.extent351-356
dc.identifierhttp://www.ncbi.nlm.nih.gov/pubmed/11333243
dc.identifier.citationGenetics, v. 158, n. 1, p. 351-356, 2001.
dc.identifier.file2-s2.0-0035024712.pdf
dc.identifier.issn0016-6731
dc.identifier.scopus2-s2.0-0035024712
dc.identifier.urihttp://hdl.handle.net/11449/66523
dc.identifier.wosWOS:000168637000031
dc.language.isoeng
dc.relation.ispartofGenetics
dc.relation.ispartofjcr4.075
dc.relation.ispartofsjr3,484
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectanimal cell
dc.subjectanimal experiment
dc.subjectblastocyst
dc.subjectBos indicus
dc.subjectBos taurus
dc.subjectcattle
dc.subjectcross breeding
dc.subjectdevelopment
dc.subjectfemale
dc.subjectgene construct
dc.subjectgenotype
dc.subjectmale
dc.subjectmating
dc.subjectmitochondrion
dc.subjectmolecular cloning
dc.subjectnonhuman
dc.subjectoocyte
dc.subjectpriority journal
dc.subjectAnimals
dc.subjectBase Sequence
dc.subjectCattle
dc.subjectCell Nucleus
dc.subjectDNA Primers
dc.subjectEmbryo
dc.subjectGenotype
dc.subjectMitochondria
dc.subjectOocytes
dc.titleComplete replacement of the mitochondrial genotype in a Bos indicus calf reconstructed by nuclear transfer to a bos taurus oocyteen
dc.typeArtigo
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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