Publicação:
A preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyrite

dc.contributor.authorFelício, A. P. [UNESP]
dc.contributor.authorDe Oliveira, E.
dc.contributor.authorOdena, M. A.
dc.contributor.authorGarcia, O. [UNESP]
dc.contributor.authorBertolini, M. C. [UNESP]
dc.contributor.authorFerraz, L. F C
dc.contributor.authorOttoboni, L. M M
dc.contributor.authorNovo, M. T M
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionProteomics Platform-Barcelona Science Park
dc.contributor.institutionUniversidade Estadual de Campinas (UNICAMP)
dc.date.accessioned2014-05-27T11:24:35Z
dc.date.available2014-05-27T11:24:35Z
dc.date.issued2009-12-28
dc.description.abstractThe Acidithiobacillus ferrooxidans periplasmic space is known to have proteins involved in the respiratory chains. There are no reports about the expression of the periplasmic proteins in A. ferrooxidans cells attached to chalcopyrite. In this preliminary work, it was compared the periplasmic protein profiles of A. ferrooxidans planktonic and attached cells after exposure to chalcopyrite for 2 hours. The bacterial response to chalcopyrite was investigated by a proteomic approach (two- dimensional gel electrophoresis and mass spectrometry). Four proteins differentially expressed between planktonic and attached cells after exposure to chalcopyrite were identified. Two of these proteins, repressed in chalcopyrite- attached cells, were both identified as superoxide dismutase, whereas the single strand binding protein (SSB) and the PspA/IM30 protein were induced. These results showed that A. ferrooxidans chalcopyrite- attached and planktonic cells show differential expression of the periplasmic proteins and that a proteomic approach can provide a valuable tool to detect proteins related to the A. ferrooxidans response to attachment to the mineral substrates. © (2009) Trans Tech Publications.en
dc.description.affiliationInstitute of Chemistry-São Paulo State University, Araraquara-SP
dc.description.affiliationProteomics Platform-Barcelona Science Park, Barcelona
dc.description.affiliationCenter of Molecular Biology and Genetic Engineering Campinas State University, Campinas-SP
dc.description.affiliationUnespInstitute of Chemistry-São Paulo State University, Araraquara-SP
dc.format.extent179-182
dc.identifierhttp://dx.doi.org/10.4028/www.scientific.net/AMR.71-73.179
dc.identifier.citationAdvanced Materials Research, v. 71-73, p. 179-182.
dc.identifier.doi10.4028/www.scientific.net/AMR.71-73.179
dc.identifier.issn1022-6680
dc.identifier.scopus2-s2.0-72449206562
dc.identifier.urihttp://hdl.handle.net/11449/71497
dc.identifier.wosWOS:000273541600038
dc.language.isoeng
dc.relation.ispartofAdvanced Materials Research
dc.relation.ispartofsjr0,121
dc.rights.accessRightsAcesso aberto
dc.sourceScopus
dc.subjectAcidithiobacillus ferrooxidans
dc.subjectBiofilm
dc.subjectChalcopyrite
dc.subjectDifferential proteomic analysis
dc.subjectPeriplasmic proteins
dc.subjectTwo-dimensional gel electrophoresis
dc.subjectDifferential expressions
dc.subjectFerrooxidans
dc.subjectMineral substrates
dc.subjectPeriplasmic space
dc.subjectPlanktonic cells
dc.subjectProteomic analysis
dc.subjectProteomic approaches
dc.subjectRespiratory chains
dc.subjectSingle-strand binding protein
dc.subjectSuper oxide dismutase
dc.subjectBiofilms
dc.subjectCopper compounds
dc.subjectElectrophoresis
dc.subjectGels
dc.subjectMass spectrometry
dc.subjectMetal recovery
dc.subjectOxygen
dc.subjectTwo dimensional
dc.subjectProteins
dc.titleA preliminary differential proteomic analysis of the periplasmic fraction in Acidithiobacillus ferrooxidans planktonic and attached cells exposed to chalcopyriteen
dc.typeTrabalho apresentado em evento
dcterms.licensehttp://www.scientific.net/AMR
dspace.entity.typePublication

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