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Identification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCR

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dc.contributor.authorCortez, A. L. L.
dc.contributor.authorCarvalho, A. C. F. B.
dc.contributor.authorIkuno, A. A.
dc.contributor.authorBurger, K. P.
dc.contributor.authorVidal-Martins, A. M. C.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T13:15:13Z
dc.date.available2014-05-20T13:15:13Z
dc.date.issued2006-12-01
dc.description.abstractThe present study was carried out to report the occurrence Salmonella spp., Salmonella Enteritidis, and Salmonella Typhimurium in chicken abattoirs. Samples of feces; feathers; scald, evisceration, and chiller water; and rinse water of non-eviscerated, eviscerated, and chilled carcass were collected from six chicken abattoirs. Salmonella isolates were identified by a multiplex-PCR using three sets of primers targeting the inuA, pefA, and sefA gene sequences from Salmonella spp., S. Typhimurium and S. Enteritidis, respectively. Salmonella spp. was detected in 10% (29/288) of the samples, whereas serovars Enteritidis and Typhimurium were identified in 62% (7/288), respectively. The results indicate the need to improve hygiene and sanitary standards in poultry slaughter lines, besides the education of food handlers and information to consumers. (c) 2006 Elsevier Ltd. All rights reserved.en
dc.description.affiliationUniv Estadual Paulista, Fac Ciências Agrarias & Vet Jaboticabal, Dept Med Vet Prevent & Reproducao Anim, BR-14884900 Jaboticabal, SP, Brazil
dc.description.affiliationInst Biol, BR-04014002 São Paulo, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Fac Ciências Agrarias & Vet Jaboticabal, Dept Med Vet Prevent & Reproducao Anim, BR-14884900 Jaboticabal, SP, Brazil
dc.format.extent340-344
dc.identifierhttp://dx.doi.org/10.1016/j.rvsc.2006.03.006
dc.identifier.citationResearch In Veterinary Science. London: W B Saunders Co Ltd, v. 81, n. 3, p. 340-344, 2006.
dc.identifier.doi10.1016/j.rvsc.2006.03.006
dc.identifier.issn0034-5288
dc.identifier.urihttp://hdl.handle.net/11449/2420
dc.identifier.wosWOS:000240951000008
dc.language.isoeng
dc.publisherW B Saunders Co Ltd
dc.relation.ispartofResearch in Veterinary Science
dc.relation.ispartofjcr1.616
dc.relation.ispartofsjr0,593
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectSalmonella spp.pt
dc.subjectSalmonella enteritidispt
dc.subjectSalmonella typhimuriumpt
dc.subjectmultiplex-PCRpt
dc.subjectchicken abattoirpt
dc.titleIdentification of Salmonella spp. isolates from chicken abattoirs by multiplex-PCRen
dc.typeArtigo
dcterms.licensehttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dcterms.rightsHolderW B Saunders Co Ltd
dspace.entity.typePublication
unesp.campusUniversidade Estadual Paulista (UNESP), Faculdade de Ciências Agrárias e Veterinárias, Jaboticabalpt
unesp.departmentMedicina Veterinária Preventiva e Reprodução Animal - FCAVpt

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Jaboticabal - FCAV - Faculdade de Ciências Agrárias e Veterinárias