Publicação: Validação de métodos para determinação do teor de 3-hidroxibutirato e 3-hidroxivalerato em polímero biodegradável
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Editor
Soc Brasileira Quimica
Tipo
Artigo
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Acesso aberto
Resumo
The efficiency of two methods for determining 3-hydroxybutyrate and 3-hydroxyvalerate content in the biopolymer (poly(3-hydroxybutyrate-co-3-hydroxyvalerate) has been evaluated. Both methods are based on (a) the hydrolysis of polymers, (b) the esterification with methyl (method A) and isoamilic alcohol (method B) and (c) analysis of the esters by gas chromatography using flame ionization detection (GC-FID). After optimization of the analytical conditions, the main validation parameters (linearity, quantification limit, detection limit, recovery, sensitivity and precision) were determined showing that both analytical procedures can be applied to control the process of poly(3-hydroxybutyrate) production. However, method B gives better analytical results than method A and has the advantage of not using chlorinated solvents.
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Palavras-chave
validation of methods, biopolymer analysis, polyhydroxyalkanoate composition
Idioma
Português
Como citar
Quimica Nova. São Paulo: Soc Brasileira Quimica, v. 30, n. 8, p. 1951-1955, 2007.
