Publicação: Spontaneous vesicles of sodium dihexadecylphosphate in HEPES buffer
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2008-04-15
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Academic Press Inc. Elsevier B.V.
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The formation of spontaneous vesicles of dihexadecylphosphate (DHP) in a HEPES buffered solution at pH 7.4, the size, morphology and melting temperature, obtained by cryogenic transmission electron microscopy (cryo-TEM) and differential scanning calorimetry (DSC), are reported. The vesicles were formed by simply mixing a 5.0 mM lipid-solvent mixture at a temperature (75 degrees C) safely above the higher melting temperature T-m = 70.4 degrees C of DHP. The vesicle diameter ranges from 100 to 332 nm and their geometry is spherical, faceted or oblong. T-m increases from 66.8 to 70.4 as DHP concentration is raised from 0.6 to 5.0 mM. (c) 2008 Elsevier B.V. All rights reserved.
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Journal of Colloid and Interface Science. San Diego: Academic Press Inc. Elsevier B.V., v. 320, n. 2, p. 608-610, 2008.
