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Crystallization, preliminary X-ray diffraction analysis and Patterson search of oxyhaemoglobin I from the wolf (Chrysocyon brachiurus)

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dc.contributor.authorSmarra, ALS
dc.contributor.authorFadel, V
dc.contributor.authorDellamano, M.
dc.contributor.authorOlivieri, JR
dc.contributor.authorde Azevedo, W. F.
dc.contributor.authorBonilla-Rodriguez, G. O.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T14:03:01Z
dc.date.available2014-05-20T14:03:01Z
dc.date.issued1999-09-01
dc.description.abstractOxyhaemoglobin I isolated from the Brazilian wolf Chrysocyon brachiurus has been crystallized and X-ray diffraction data has been collected to 2.06 A resolution using a synchrotron-radiation source. Crystals were determined to belong to the space group P2(1)2(1)2(1) and preliminary structural analysis revealed the presence of one tetramer in the asymmetric unit. The structure was determined using standard molecular-replacement techniques and is currently being refined using maximum-likelihood protocols. This is the first haemoglobin isolated from a member of the Canidae family to be crystallized and it will provide further insights in the comparative biochemistry of vertebrate haemoglobins.en
dc.description.affiliationUNESP, Dept Quim & Geociencias, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Quim & Geociencias, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.format.extent1618-1619
dc.identifierhttp://dx.doi.org/10.1107/S0907444999009725
dc.identifier.citationActa Crystallographica Section D-biological Crystallography. Copenhagen: Munksgaard Int Publ Ltd, v. 55, p. 1618-1619, 1999.
dc.identifier.doi10.1107/S0907444999009725
dc.identifier.fileWOS000082727800025.pdf
dc.identifier.issn0907-4449
dc.identifier.lattes2835029061696580
dc.identifier.lattes2406867656111498
dc.identifier.urihttp://hdl.handle.net/11449/22204
dc.identifier.wosWOS:000082727800025
dc.language.isoeng
dc.publisherMunksgaard Int Publ Ltd
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallography
dc.rights.accessRightsAcesso aberto
dc.sourceWeb of Science
dc.titleCrystallization, preliminary X-ray diffraction analysis and Patterson search of oxyhaemoglobin I from the wolf (Chrysocyon brachiurus)en
dc.typeArtigo
dcterms.licensehttp://journals.iucr.org/services/copyrightpolicy.html
dcterms.rightsHolderMunksgaard Int Publ Ltd
dspace.entity.typePublication
unesp.author.lattes2835029061696580
unesp.author.lattes2406867656111498
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentQuímica e Ciências Ambientais - IBILCEpt

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São José do Rio Preto - IBILCE - Instituto de Biociências, Letras e Ciências Exatas