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Purification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ : 1501)

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dc.contributor.authorGomes, E.
dc.contributor.authorSerzedello, A.
dc.contributor.authorDa Silva, R.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.date.accessioned2014-05-20T13:56:05Z
dc.date.available2014-05-20T13:56:05Z
dc.date.issued1998-01-01
dc.description.abstractA RNase of Aspergillus flavipes (IZ:1501) was purified from culture medium by chromatography on DEAE-cellulose and Sephadex G50 columns, after 96 h of cultivation. The molecular weight of the RNase was estimated to be 15 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 55 degrees C, respectively. Catalytic activity was inhibited by Hg2+, Ag+, Fe3+, Co2+ and Zn2+. The enzyme showed guanosine specificity producing only 3'-GMP from yeast RNA.en
dc.description.affiliationUniv Estadual Paulista, Dept Biol, Lab Bioquim Proc & Microbiol Aplicada, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUniv Estadual Paulista, Dept Quim & Geosci, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUNESP, Dept Bioquim & Microbiol, Rio Claro, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Biol, Lab Bioquim Proc & Microbiol Aplicada, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Dept Quim & Geosci, IBILCE, BR-15054000 Sao Jose do Rio Preto, SP, Brazil
dc.description.affiliationUnespUNESP, Dept Bioquim & Microbiol, Rio Claro, SP, Brazil
dc.format.extent39-50
dc.identifierhttp://cat.inist.fr/?aModele=afficheN&cpsidt=1709661
dc.identifier.citationMicrobios. Cambridge: Faculty Press, v. 96, n. 383, p. 39-50, 1998.
dc.identifier.issn0026-2633
dc.identifier.lattes7091241742851920
dc.identifier.lattes9424175688206545
dc.identifier.urihttp://hdl.handle.net/11449/20060
dc.identifier.wosWOS:000078475500004
dc.language.isoeng
dc.publisherFaculty Press
dc.relation.ispartofMicrobios
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectribonucleasept
dc.subjectAspergilluspt
dc.subjectnucleotidespt
dc.titlePurification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ : 1501)en
dc.typeArtigo
dcterms.rightsHolderFaculty Press
dspace.entity.typePublication
unesp.author.lattes7091241742851920
unesp.author.lattes9424175688206545
unesp.author.orcid0000-0003-1468-5752[3]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Rio Claropt
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Biociências, Letras e Ciências Exatas, São José do Rio Pretopt
unesp.departmentBioquímica e Microbiologia - IBpt
unesp.departmentQuímica e Ciências Ambientais - IBILCEpt

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São José do Rio Preto - IBILCE - Instituto de Biociências, Letras e Ciências Exatas
Rio Claro - IB - Instituto de Biociências