Publicação: Application of HPLC-NMR coupling using C30 phase in the separation and identification of flavonoids of taxonomic relevance
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The on-line separation and identification of two important taxonomic markers for plant species of the Paepalanthus genus, the flavonoids 6-methoxykaempferol-3-O-β-D-glucopyranoside and 6-methoxykaempferol-3-O-β-D-6″(p-coumaroyl)glucopyranoside, has been performed with an HPLC-NMR coupling using C30 phase. 1D spectra have been recorded in the stopped-flow mode for the two predominant chromatographic peaks. This is the first application of HPLC-NMR coupling using C30 phase to a taxonomic problem. The technique drastically reduces the required amount of sampling for structure determination. © Springer-Verlag 2000.
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flavonoid, chemistry, classification, equipment design, high performance liquid chromatography, isolation and purification, medicinal plant, methodology, nuclear magnetic resonance spectroscopy, online system, standard, Chromatography, High Pressure Liquid, Equipment Design, Flavonoids, Magnetic Resonance Spectroscopy, Online Systems, Plants, Medicinal
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Inglês
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Fresenius' Journal of Analytical Chemistry, v. 368, n. 5, p. 540-542, 2000.
