Application of HPLC-NMR coupling using C30 phase in the separation and identification of flavonoids of taxonomic relevance
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Springer Nature
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The on-line separation and identification of two important taxonomic markers for plant species of the Paepalanthus genus, the flavonoids 6-methoxykaempferol-3-O-β-D-glucopyranoside and 6-methoxykaempferol-3-O-β-D-6″(p-coumaroyl)glucopyranoside, has been performed with an HPLC-NMR coupling using C30 phase. 1D spectra have been recorded in the stopped-flow mode for the two predominant chromatographic peaks. This is the first application of HPLC-NMR coupling using C30 phase to a taxonomic problem. The technique drastically reduces the required amount of sampling for structure determination. © Springer-Verlag 2000.
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flavonoid , chemistry , classification , equipment design , high performance liquid chromatography , isolation and purification , medicinal plant , methodology , nuclear magnetic resonance spectroscopy , online system , standard , Chromatography, High Pressure Liquid , Equipment Design , Flavonoids , Magnetic Resonance Spectroscopy , Online Systems , Plants, Medicinal
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Inglês
Citação
Fresenius' Journal of Analytical Chemistry, v. 368, n. 5, p. 540-542, 2000.
