Pig liver esterase (PLE) extraction.
| dc.contributor.author | Trevisan, Henrique Celso | |
| dc.contributor.author | Medeiros, Joao Batista de | |
| dc.contributor.author | Favero Lisboa, Helen Cristina | |
| dc.contributor.institution | Universidade Estadual Paulista (Unesp) | |
| dc.date.accessioned | 2020-12-10T18:05:35Z | |
| dc.date.available | 2020-12-10T18:05:35Z | |
| dc.date.issued | 2006-07-01 | |
| dc.description.abstract | A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 W of PLE suspension were obtained after 8 hours, at an estimated cost of US$0.21/kU. The PLE concentrate thus obtained was stable, showing 96-100% of the initial activity after 7 months in refrigerator at 4 degrees C. | en |
| dc.description.affiliation | Univ Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14800900 Araraquara, SP, Brazil | |
| dc.description.affiliationUnesp | Univ Estadual Paulista, Inst Quim, Dept Bioquim & Tecnol Quim, BR-14800900 Araraquara, SP, Brazil | |
| dc.format.extent | 865-867 | |
| dc.identifier.citation | Quimica Nova. Sao Paulo: Soc Brasileira Quimica, v. 29, n. 4, p. 865-867, 2006. | |
| dc.identifier.issn | 0100-4042 | |
| dc.identifier.uri | http://hdl.handle.net/11449/195856 | |
| dc.identifier.wos | WOS:000239388400040 | |
| dc.language.iso | por | |
| dc.publisher | Soc Brasileira Quimica | |
| dc.relation.ispartof | Quimica Nova | |
| dc.source | Web of Science | |
| dc.subject | porcine liver esterase | |
| dc.subject | PLE | |
| dc.subject | biocatalysis | |
| dc.title | Pig liver esterase (PLE) extraction. | en |
| dc.type | Artigo | pt |
| dcterms.rightsHolder | Soc Brasileira Quimica | |
| dspace.entity.type | Publication | |
| relation.isOrgUnitOfPublication | bc74a1ce-4c4c-4dad-8378-83962d76c4fd | |
| relation.isOrgUnitOfPublication.latestForDiscovery | bc74a1ce-4c4c-4dad-8378-83962d76c4fd | |
| unesp.campus | Universidade Estadual Paulista (UNESP), Instituto de Química, Araraquara | pt |
| unesp.department | Bioquímica e Tecnologia - IQ | pt |