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Semliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals production

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dc.contributor.authorNúñez, Eutimio Gustavo Fernández [UNESP]
dc.contributor.authorJorge, Soraia Attie Calil
dc.contributor.authorAstray, Renato Mancini
dc.contributor.authorde Rezende, Alexandre Gonçalves
dc.contributor.authorda Costa, Bruno Labate Vale
dc.contributor.authorMonteiro, Daniella Cristina Ventini
dc.contributor.authorPereira, Carlos Augusto
dc.contributor.authorTonso, Aldo
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.contributor.institutionUniversidade Estadual Paulista (UNESP)
dc.contributor.institutionInstituto Butantan
dc.contributor.institutionInstituto de Pesquisas Tecnológicas do Estado de São Paulo
dc.date.accessioned2022-04-29T07:12:26Z
dc.date.available2022-04-29T07:12:26Z
dc.date.issued2013-09-01
dc.description.abstractThe number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale.en
dc.description.affiliationLaboratório de Células Animais Departamento de Engenharia Química, Escola Politécnica Universidade de São Paulo, São Paulo SP
dc.description.affiliationDepartamento de Ciências Biológicas Faculdade de Ciência e Letras Universidade Estadual Paulista Julho de Mesquita Filho, Assis SP
dc.description.affiliationLaboratório de Imunologia Viral Instituto Butantan, São Paulo SP
dc.description.affiliationLaboratório de Biotecnologia Industrial Núcleo de Bionanomanufatura Instituto de Pesquisas Tecnológicas do Estado de São Paulo, São Paulo SP
dc.description.affiliationUnespDepartamento de Ciências Biológicas Faculdade de Ciência e Letras Universidade Estadual Paulista Julho de Mesquita Filho, Assis SP
dc.format.extent859-866
dc.identifierhttp://dx.doi.org/10.1590/S1516-89132013000500018
dc.identifier.citationBrazilian Archives of Biology and Technology, v. 56, n. 5, p. 859-866, 2013.
dc.identifier.doi10.1590/S1516-89132013000500018
dc.identifier.issn1516-8913
dc.identifier.issn1678-4324
dc.identifier.scopus2-s2.0-84887180324
dc.identifier.urihttp://hdl.handle.net/11449/227294
dc.language.isoeng
dc.relation.ispartofBrazilian Archives of Biology and Technology
dc.sourceScopus
dc.subjectBHK-21
dc.subjectLarge-scale bioprocesses
dc.subjectMammalian cells
dc.subjectRecombinant proteins
dc.subjectSFV
dc.subjectTransient expression
dc.titleSemliki forest virus as a vector: Pros and cons for its use in biopharmaceuticals productionen
dc.typeArtigo
dspace.entity.typePublication
unesp.departmentCiências Biológicas - FCLASpt

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