A DNA tool for the identification of heavily exploited Atlantic billfishes
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Due to the morphological similarities among species, the International Commission for the Conservation of the Atlantic Tunas has recommended the use of diagnostic molecular tools to allow for robust species-level identifications of the billfishes. In this study, a protocol for the molecular identification of all six Atlantic billfishes was developed utilizing a PCR–RFLP approach, targeting the mitochondrial gene cytochrome c oxidase subunit 1. A survey of 28 restriction endonucleases identified two enzymes (TaqI and HaeIII) that produced species-specific banding patterns sufficient to distinguish species. The protocol was validated against billfishes captured across their Atlantic distributions.
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COI, Fisheries management, Genetics, PCR–RFLP, Pelagic fish
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Inglês
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Conservation Genetics Resources, v. 7, n. 4, p. 807-809, 2015.
