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Validated HPLC method for the standardization of Phyllanthus niruri (herb and commercial extracts) using corilagin as a phytochemical marker

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dc.contributor.authorColombo, Renata [UNESP]
dc.contributor.authorBatista, Andrea N. de L. [UNESP]
dc.contributor.authorTeles, Helder L. [UNESP]
dc.contributor.authorSilva, Geraldo H. [UNESP]
dc.contributor.authorBomfim, Giovani C. C. [UNESP]
dc.contributor.authorBurgos, Rosilene C. R. [UNESP]
dc.contributor.authorCavalheiro, Alberto José [UNESP]
dc.contributor.authorBolzani, Vanderlan da Silva [UNESP]
dc.contributor.authorSilva, Dulce Helena Siqueira [UNESP]
dc.contributor.authorPelicia, Clicia R.
dc.contributor.authorGuimaraes, Fabiana M.
dc.contributor.authorHeimberg, Maria Celia H.
dc.contributor.institutionUniversidade Estadual Paulista (Unesp)
dc.contributor.institutionUniversidade de São Paulo (USP)
dc.date.accessioned2014-05-20T14:20:13Z
dc.date.available2014-05-20T14:20:13Z
dc.date.issued2009-06-01
dc.description.abstractPhyllanthus niruri L., commonly known in Brazil as 'quebra-pedra', has long been used in the treatment of diverse diseases and especially urolithiasis. The therapeutic effects of P. niruri are attributed to various compounds present in the plant, including the hydrolysable tannin corilagin. In the present study, high-performance liquid chromatography (HPLC-/PAD) profiles of leaves and commercial extracts of P. niruri were examined and three compounds, found to be present in all of the samples studied, were isolated by open column chromatography over C,8 silica gel followed by preparative HPLC. These compounds were identified by nuclear magnetic resonance as corilagin, rutin and ethyl 3,4,5-trihydroxybenzoate. Corilagin, which has been proposed as a phytochemical marker for P. niruri, was employed as an external standard in the development and validation of a rapid and efficient qualitative and quantitative HPLC assay for the analyte. The method may be applied in the standardization of herbs and phytomedicines commercialized in Brazil as quebra-pedra. Copyright (C) 2009 John Wiley & Sons, Ltd.en
dc.description.affiliationUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil
dc.description.affiliationAnidro Brasil Extracoes Ltda, Botucatu, SP, Brazil
dc.description.affiliationUnespUniv Estadual Paulista, Inst Quim, BR-14800900 Araraquara, SP, Brazil
dc.description.sponsorshipConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.description.sponsorshipIdCNPq: 500185/2006-9
dc.format.extent573-580
dc.identifierhttp://dx.doi.org/10.1002/bmc.1155
dc.identifier.citationBiomedical Chromatography. Chichester: John Wiley & Sons Ltd, v. 23, n. 6, p. 573-580, 2009.
dc.identifier.doi10.1002/bmc.1155
dc.identifier.issn0269-3879
dc.identifier.lattes2518006820764120
dc.identifier.lattes4484083685251673
dc.identifier.lattes4702004904231248
dc.identifier.orcid0000-0002-1516-7765
dc.identifier.urihttp://hdl.handle.net/11449/26075
dc.identifier.wosWOS:000266182600003
dc.language.isoeng
dc.publisherJohn Wiley & Sons Ltd
dc.relation.ispartofBiomedical Chromatography
dc.relation.ispartofjcr1.688
dc.relation.ispartofsjr0,490
dc.rights.accessRightsAcesso restrito
dc.sourceWeb of Science
dc.subjectPhyllanthus nirurien
dc.subjectcorilaginen
dc.subjectHPLC-PADen
dc.subjectstandardizationen
dc.subjectquebra-pedraen
dc.titleValidated HPLC method for the standardization of Phyllanthus niruri (herb and commercial extracts) using corilagin as a phytochemical markeren
dc.typeArtigo
dcterms.licensehttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dcterms.rightsHolderJohn Wiley & Sons Ltd
dspace.entity.typePublication
unesp.author.lattes2518006820764120[7]
unesp.author.lattes4484083685251673
unesp.author.lattes4702004904231248[9]
unesp.author.orcid0000-0002-1516-7765[9]
unesp.author.orcid0000-0001-8214-9957[7]
unesp.campusUniversidade Estadual Paulista (UNESP), Instituto de Química, Araraquarapt
unesp.departmentQuímica Orgânica - IQARpt

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Araraquara - IQAR - Instituto de Química